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Literature summary extracted from

  • Yokoyama, K.; Yamamoto, Y.; Kudo, F.; Eguchi, T.
    Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis (2008), ChemBioChem, 9, 865-869.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.283 co-expressed with molecular chaperone GroES and GroEL in Escherichia coli BL21(DE3) cells Streptomyces sp.
2.4.1.283 expressed as a His-tagged fusion protein in Escherichia coli Streptomyces fradiae
2.4.1.285 expressed as a His-tagged fusion protein in Escherichia coli Streptomyces fradiae
2.4.1.285 expressed in Escherichia coli BL21(DE3) cells Streptomyces sp.
3.5.1.112 expressed as a His-tagged fusion protein in Escherichia coli Streptomyces fradiae
3.5.1.113 expressed as a His-tagged fusion protein in Escherichia coli Streptomyces fradiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.285 Ca2+ about 55% residual activity in the presence of 2 mM Streptomyces sp.
2.4.1.285 Co2+ about 90% residual activity in the presence of 2 mM Streptomyces sp.
2.4.1.285 Cu2+ inhibits activity Streptomyces fradiae
2.4.1.285 Cu2+ complete inhibition in the presence of 2 mM Streptomyces sp.
2.4.1.285 EDTA about 20% residual activity in the presence of 10 mM Streptomyces sp.
2.4.1.285 Ni2+ about 80% residual activity in the presence of 2 mM Streptomyces sp.
2.4.1.285 Zn2+ inhibits activity Streptomyces fradiae
2.4.1.285 Zn2+ complete inhibition in the presence of 2 mM Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.285 Mg2+
-
 Streptomyces fradiae
2.4.1.285 Mg2+ 100% activity in the presence of 2 mM Streptomyces sp.
2.4.1.285 Mn2+
-
 Streptomyces fradiae
2.4.1.285 Mn2+ 105% activity in the presence of 2 mM Mn2+ compared to Mg2+ Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.283 Streptomyces fradiae
-
-
-
2.4.1.283 Streptomyces sp.
-
-
-
2.4.1.285 Streptomyces fradiae
-
-
-
2.4.1.285 Streptomyces sp.
-
-
-
3.5.1.112 Streptomyces fradiae
-
-
-
3.5.1.113 Streptomyces fradiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.283 a poor amount is isolated using Ni-NTA chromatography. The cell-free extract of Escherichia coli expressing NeoD is used for enzymatic assays Streptomyces fradiae
2.4.1.285 DEAE Sepharose column chromatography, Superdex Hi-Load 200 gel filtration, and Mono Q column chromatography Streptomyces sp.
2.4.1.285 purified to homogeneity by DEAE Sepharose column chromatography, gel filtration, and MonoQ column chromatography Streptomyces fradiae
3.5.1.112 purified with TALON cobalt affinity resin Streptomyces fradiae
3.5.1.113 purified with TALON cobalt affinity resin Streptomyces fradiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.283 additional information other possible glycosyl donor, UDP-glucose, and other possible glycosyl acceptors, paromamine, neamine, and ribostamycin are not accepted Streptomyces fradiae ?
-
 ?
2.4.1.283 additional information the enzyme is unable to catalyze the glycosylation of ribostamycin Streptomyces sp. ?
-
 ?
2.4.1.283 UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine
-
 Streptomyces fradiae UDP + 2'-N-acetylparomamine
-
 ?
2.4.1.283 UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine about 80% of 2-deoxystreptamine is conversed Streptomyces sp. UDP + 2'-N-acetylparomamine
-
 ?
2.4.1.285 UDP-N-acetyl-alpha-D-glucosamine + ribostamycin
-
 Streptomyces sp. UDP + 2'''-acetyl-6'''-hydroxyneomycin C
-
 ?
2.4.1.285 UDP-N-acetyl-alpha-D-glucosamine + ribostamycin
-
 Streptomyces fradiae UDP + 2'''-acetyl-6'''-hydroxyneomycin C
-
 ?
3.5.1.112 N-acetylparomamine + H2O
-
 Streptomyces fradiae paromamine + acetate
-
 ?
3.5.1.113 2'''-N-acetyl-6'''-deamino-6'''-hydroxyneomycin C + H2O
-
 Streptomyces fradiae 6'''-deamino-6'''-hydroxyneomycin C
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.283 neoD
-
 Streptomyces sp.
2.4.1.283 neoD
-
 Streptomyces fradiae
2.4.1.285 Neo15
-
 Streptomyces sp.
2.4.1.285 neoK
-
 Streptomyces sp.
2.4.1.285 neoK
-
 Streptomyces fradiae
3.5.1.112 neoL
-
 Streptomyces fradiae
3.5.1.113 neoL
-
 Streptomyces fradiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.283 28
-
 assay at Streptomyces fradiae
2.4.1.285 28
-
 assay at Streptomyces fradiae
3.5.1.112 28
-
 assay at Streptomyces fradiae
3.5.1.113 28
-
 assay at Streptomyces fradiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.283 7
-
 assay at Streptomyces fradiae
2.4.1.285 7
-
 assay at Streptomyces fradiae
3.5.1.112 7
-
 assay at Streptomyces fradiae
3.5.1.113 7
-
 assay at Streptomyces fradiae

General Information

EC Number General Information Comment Organism
2.4.1.285 metabolism NeoK is a glycosyltransferase that catalyzes the retaining glycosylation of ribostamycin with UDP-GlcNAc as a sugar donor Streptomyces fradiae