BRENDA - Enzyme Database

Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin

Huang, F.; Spiteller, D.; Koorbanally, N.A.; Li, Y.; Llewellyn, N.M.; Spencer, J.B.; ChemBioChem 8, 283-288 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.1.3.43
expression in Escherichia coli
Streptomyces fradiae
1.1.3.44
expression in Escherichia coli
Streptomyces fradiae
2.6.1.93
expressed in Escherichia coli BL21(DE3) cells
Bacillus circulans
2.6.1.93
expressed in Escherichia coli BL21(DE3) cells
Streptomyces fradiae
2.6.1.95
expressed in Escherichia coli BL21(DE3) cells
Streptomyces fradiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.43
60034
-
x * 60034, LC-ESI-MS
Streptomyces fradiae
1.1.3.44
60034
-
x * 60034, LC-ESI-MS
Streptomyces fradiae
2.6.1.93
48060
-
Neo-18, calculated from amino acid sequence
Streptomyces fradiae
2.6.1.93
48060
-
Neo-18, electrospray ionization mass spectrometry
Streptomyces fradiae
2.6.1.93
52070
-
BtrB, calculated from amino acid sequence
Bacillus circulans
2.6.1.93
52070
-
BtrB, electrospray ionization mass spectrometry
Bacillus circulans
2.6.1.95
48060
-
Neo-18, calculated from amino acid sequence
Streptomyces fradiae
2.6.1.95
48060
-
Neo-18, electrospray ionization mass spectrometry
Streptomyces fradiae
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.3.43
Streptomyces fradiae
Q53U15
-
-
1.1.3.43
Streptomyces fradiae NCIMB 8233
Q53U15
-
-
1.1.3.44
Streptomyces fradiae
Q53U15
-
-
1.1.3.44
Streptomyces fradiae NCIMB 8233
Q53U15
-
-
2.6.1.93
Bacillus circulans
-
-
-
2.6.1.93
Bacillus circulans B3312
-
-
-
2.6.1.93
Streptomyces fradiae
-
-
-
2.6.1.93
Streptomyces fradiae NCIMB 8233
-
-
-
2.6.1.95
Streptomyces fradiae
-
-
-
2.6.1.95
Streptomyces fradiae NCIMB 8233
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.6.1.93
Ni2+-NTA resin column chromatography
Bacillus circulans
2.6.1.93
Ni2+-NTA resin column chromatography
Streptomyces fradiae
2.6.1.95
Ni2+-NTA resin column chromatography
Streptomyces fradiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.3.43
additional information
enzyme also catalyzes reaction of EC 1.1.3.44, 6''-hydroxyneomycin C oxidase
719294
Streptomyces fradiae
?
-
-
-
?
1.1.3.43
additional information
enzyme also catalyzes reaction of EC 1.1.3.44, 6''-hydroxyneomycin C oxidase
719294
Streptomyces fradiae NCIMB 8233
?
-
-
-
?
1.1.3.43
paromamine + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae
6'-dehydro-paromamine + H2O2
-
-
-
?
1.1.3.43
paromamine + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae NCIMB 8233
6'-dehydro-paromamine + H2O2
-
-
-
?
1.1.3.44
6'''-deamino-6'''-hydroxyneomycin C + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae
6'''-deamino-6'''-oxoneomycin C + H2O2
-
-
-
?
1.1.3.44
6'''-deamino-6'''-hydroxyneomycin C + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae NCIMB 8233
6'''-deamino-6'''-oxoneomycin C + H2O2
-
-
-
?
1.1.3.44
additional information
enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
719294
Streptomyces fradiae
?
-
-
-
?
1.1.3.44
additional information
enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
719294
Streptomyces fradiae NCIMB 8233
?
-
-
-
?
2.6.1.93
6'-dehydroparomamine + L-glutamate
-
719294
Bacillus circulans
neamine + 2-oxoglutarate
-
-
-
?
2.6.1.93
6'-dehydroparomamine + L-glutamate
-
719294
Streptomyces fradiae
neamine + 2-oxoglutarate
-
-
-
?
2.6.1.93
6'-dehydroparomamine + L-glutamate
-
719294
Streptomyces fradiae NCIMB 8233
neamine + 2-oxoglutarate
-
-
-
?
2.6.1.93
6'-dehydroparomamine + L-glutamate
-
719294
Bacillus circulans B3312
neamine + 2-oxoglutarate
-
-
-
?
2.6.1.95
neomycin + 2-oxoglutarate
-
719294
Streptomyces fradiae
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
-
-
?
2.6.1.95
neomycin + 2-oxoglutarate
-
719294
Streptomyces fradiae NCIMB 8233
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.43
?
x * 60034, LC-ESI-MS
Streptomyces fradiae
1.1.3.44
?
x * 60034, LC-ESI-MS
Streptomyces fradiae
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.3.44
Neo-11
-
Streptomyces fradiae
2.6.1.93
6'-oxoglucosaminyl:L-glutamate aminotransferase
-
Bacillus circulans
2.6.1.93
6'-oxoglucosaminyl:L-glutamate aminotransferase
-
Streptomyces fradiae
2.6.1.93
btrB
-
Bacillus circulans
2.6.1.93
Neo-18
-
Streptomyces fradiae
2.6.1.95
6'-oxoglucosaminyl:L-glutamate aminotransferase
-
Streptomyces fradiae
2.6.1.95
Neo-18
-
Streptomyces fradiae
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.43
30
-
assay at
Streptomyces fradiae
1.1.3.44
30
-
assay at
Streptomyces fradiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.43
8
-
assay at
Streptomyces fradiae
1.1.3.44
8
-
assay at
Streptomyces fradiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.43
FAD
-
Streptomyces fradiae
1.1.3.44
FAD
-
Streptomyces fradiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.43
expression in Escherichia coli
Streptomyces fradiae
1.1.3.44
expression in Escherichia coli
Streptomyces fradiae
2.6.1.93
expressed in Escherichia coli BL21(DE3) cells
Bacillus circulans
2.6.1.93
expressed in Escherichia coli BL21(DE3) cells
Streptomyces fradiae
2.6.1.95
expressed in Escherichia coli BL21(DE3) cells
Streptomyces fradiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.43
FAD
-
Streptomyces fradiae
1.1.3.44
FAD
-
Streptomyces fradiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.43
60034
-
x * 60034, LC-ESI-MS
Streptomyces fradiae
1.1.3.44
60034
-
x * 60034, LC-ESI-MS
Streptomyces fradiae
2.6.1.93
48060
-
Neo-18, calculated from amino acid sequence
Streptomyces fradiae
2.6.1.93
48060
-
Neo-18, electrospray ionization mass spectrometry
Streptomyces fradiae
2.6.1.93
52070
-
BtrB, calculated from amino acid sequence
Bacillus circulans
2.6.1.93
52070
-
BtrB, electrospray ionization mass spectrometry
Bacillus circulans
2.6.1.95
48060
-
Neo-18, calculated from amino acid sequence
Streptomyces fradiae
2.6.1.95
48060
-
Neo-18, electrospray ionization mass spectrometry
Streptomyces fradiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.93
Ni2+-NTA resin column chromatography
Bacillus circulans
2.6.1.93
Ni2+-NTA resin column chromatography
Streptomyces fradiae
2.6.1.95
Ni2+-NTA resin column chromatography
Streptomyces fradiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.3.43
additional information
enzyme also catalyzes reaction of EC 1.1.3.44, 6''-hydroxyneomycin C oxidase
719294
Streptomyces fradiae
?
-
-
-
?
1.1.3.43
additional information
enzyme also catalyzes reaction of EC 1.1.3.44, 6''-hydroxyneomycin C oxidase
719294
Streptomyces fradiae NCIMB 8233
?
-
-
-
?
1.1.3.43
paromamine + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae
6'-dehydro-paromamine + H2O2
-
-
-
?
1.1.3.43
paromamine + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae NCIMB 8233
6'-dehydro-paromamine + H2O2
-
-
-
?
1.1.3.44
6'''-deamino-6'''-hydroxyneomycin C + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae
6'''-deamino-6'''-oxoneomycin C + H2O2
-
-
-
?
1.1.3.44
6'''-deamino-6'''-hydroxyneomycin C + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae NCIMB 8233
6'''-deamino-6'''-oxoneomycin C + H2O2
-
-
-
?
1.1.3.44
additional information
enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
719294
Streptomyces fradiae
?
-
-
-
?
1.1.3.44
additional information
enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
719294
Streptomyces fradiae NCIMB 8233
?
-
-
-
?
2.6.1.93
6'-dehydroparomamine + L-glutamate
-
719294
Bacillus circulans
neamine + 2-oxoglutarate
-
-
-
?
2.6.1.93
6'-dehydroparomamine + L-glutamate
-
719294
Streptomyces fradiae
neamine + 2-oxoglutarate
-
-
-
?
2.6.1.93
6'-dehydroparomamine + L-glutamate
-
719294
Streptomyces fradiae NCIMB 8233
neamine + 2-oxoglutarate
-
-
-
?
2.6.1.93
6'-dehydroparomamine + L-glutamate
-
719294
Bacillus circulans B3312
neamine + 2-oxoglutarate
-
-
-
?
2.6.1.95
neomycin + 2-oxoglutarate
-
719294
Streptomyces fradiae
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
-
-
?
2.6.1.95
neomycin + 2-oxoglutarate
-
719294
Streptomyces fradiae NCIMB 8233
6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.43
?
x * 60034, LC-ESI-MS
Streptomyces fradiae
1.1.3.44
?
x * 60034, LC-ESI-MS
Streptomyces fradiae
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.43
30
-
assay at
Streptomyces fradiae
1.1.3.44
30
-
assay at
Streptomyces fradiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.43
8
-
assay at
Streptomyces fradiae
1.1.3.44
8
-
assay at
Streptomyces fradiae
General Information
EC Number
General Information
Commentary
Organism
1.1.3.43
physiological function
joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5’-phosphate-mediated transamination
Streptomyces fradiae
1.1.3.44
physiological function
joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination
Streptomyces fradiae
2.6.1.93
metabolism
BtrB is involved in neamine biosynthesis
Bacillus circulans
2.6.1.93
metabolism
Neo-18 is involved in neamine biosynthesis
Streptomyces fradiae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.3.43
physiological function
joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5’-phosphate-mediated transamination
Streptomyces fradiae
1.1.3.44
physiological function
joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination
Streptomyces fradiae
2.6.1.93
metabolism
BtrB is involved in neamine biosynthesis
Bacillus circulans
2.6.1.93
metabolism
Neo-18 is involved in neamine biosynthesis
Streptomyces fradiae