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Literature summary extracted from

  • Unsoeld, I.A.; Li, S.M.
    Reverse prenyltransferase in the biosynthesis of fumigaclavine C in Aspergillus fumigatus: gene expression, purification, and characterization of fumigaclavine C synthase FGAPT1 (2006), ChemBioChem, 7, 158-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.100 gene fgaPT1, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.100 Mn2+ 90% inhibition at 5 mM Aspergillus fumigatus
2.5.1.100 Zn2+ 90% inhibition at 5 mM Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.100 0.006
-
 fumigaclavine A pH 7.5, 30°C Aspergillus fumigatus
2.5.1.100 0.013
-
 dimethylallyl diphosphate pH 7.5, 30°C Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.100 soluble
-
 Aspergillus fumigatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.100 additional information no significant effects by Mg2+, Ca2+, and EDTA at 5 mM Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.100 110000
-
 recombinant His6-tagged FgaPT1, gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.100 fumigaclavine A + dimethylallyl diphosphate Aspergillus fumigatus
-
 fumigaclavine C + diphosphate
-
 ?
2.5.1.100 fumigaclavine A + dimethylallyl diphosphate Aspergillus fumigatus Af293
-
 fumigaclavine C + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.100 Aspergillus fumigatus
-
 gene fgaPT1
-
2.5.1.100 Aspergillus fumigatus Af293
-
 gene fgaPT1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.100 recombinant His6-tagged FgaPT1 from Escherichia coli strain BL21(DE3)pLysS to near homogeneity Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.100 fumigaclavine A + dimethylallyl diphosphate
-
 Aspergillus fumigatus fumigaclavine C + diphosphate
-
 ?
2.5.1.100 fumigaclavine A + dimethylallyl diphosphate the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus Aspergillus fumigatus fumigaclavine C + diphosphate
-
 ?
2.5.1.100 fumigaclavine A + dimethylallyl diphosphate
-
 Aspergillus fumigatus Af293 fumigaclavine C + diphosphate
-
 ?
2.5.1.100 fumigaclavine A + dimethylallyl diphosphate the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus Aspergillus fumigatus Af293 fumigaclavine C + diphosphate
-
 ?
2.5.1.100 additional information FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A Aspergillus fumigatus ?
-
 ?
2.5.1.100 additional information FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A Aspergillus fumigatus Af293 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.100 dimer 2 x 53000, recombinant His6-tagged FgaPT1, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.100 FgaPT1
-
 Aspergillus fumigatus
2.5.1.100 fumigaclavine C synthase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.100 30
-
 assay at Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.100 0.8
-
 dimethylallyl diphosphate pH 7.5, 30°C Aspergillus fumigatus
2.5.1.100 0.8
-
 fumigaclavine A pH 7.5, 30°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.100 7.5
-
 assay at Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.5.1.100 evolution FgaPT1 shows very low sequence similarity to the recently identified prenyltransferase LtxC from cyanobacteria, which also catalyzes the reverse prenylation of an indole nucleus Aspergillus fumigatus