BRENDA - Enzyme Database

Reverse prenyltransferase in the biosynthesis of fumigaclavine C in Aspergillus fumigatus: gene expression, purification, and characterization of fumigaclavine C synthase FGAPT1

Unsoeld, I.A.; Li, S.M.; ChemBioChem 7, 158-164 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.5.1.100
gene fgaPT1, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
Aspergillus fumigatus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.100
Mn2+
90% inhibition at 5 mM
Aspergillus fumigatus
2.5.1.100
Zn2+
90% inhibition at 5 mM
Aspergillus fumigatus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.100
0.006
-
fumigaclavine A
pH 7.5, 30°C
Aspergillus fumigatus
2.5.1.100
0.013
-
dimethylallyl diphosphate
pH 7.5, 30°C
Aspergillus fumigatus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.5.1.100
soluble
-
Aspergillus fumigatus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.5.1.100
additional information
no significant effects by Mg2+, Ca2+, and EDTA at 5 mM
Aspergillus fumigatus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.100
110000
-
recombinant His6-tagged FgaPT1, gel filtration
Aspergillus fumigatus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
Aspergillus fumigatus
-
fumigaclavine C + diphosphate
-
-
?
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
Aspergillus fumigatus Af293
-
fumigaclavine C + diphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.100
Aspergillus fumigatus
-
gene fgaPT1
-
2.5.1.100
Aspergillus fumigatus Af293
-
gene fgaPT1
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.100
recombinant His6-tagged FgaPT1 from Escherichia coli strain BL21(DE3)pLysS to near homogeneity
Aspergillus fumigatus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
-
719293
Aspergillus fumigatus
fumigaclavine C + diphosphate
-
-
-
?
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus
719293
Aspergillus fumigatus
fumigaclavine C + diphosphate
-
-
-
?
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
-
719293
Aspergillus fumigatus Af293
fumigaclavine C + diphosphate
-
-
-
?
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus
719293
Aspergillus fumigatus Af293
fumigaclavine C + diphosphate
-
-
-
?
2.5.1.100
additional information
FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A
719293
Aspergillus fumigatus
?
-
-
-
-
2.5.1.100
additional information
FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A
719293
Aspergillus fumigatus Af293
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.5.1.100
dimer
2 x 53000, recombinant His6-tagged FgaPT1, SDS-PAGE
Aspergillus fumigatus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.100
30
-
assay at
Aspergillus fumigatus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.100
0.8
-
dimethylallyl diphosphate
pH 7.5, 30°C
Aspergillus fumigatus
2.5.1.100
0.8
-
fumigaclavine A
pH 7.5, 30°C
Aspergillus fumigatus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.100
7.5
-
assay at
Aspergillus fumigatus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.100
gene fgaPT1, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
Aspergillus fumigatus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.100
Mn2+
90% inhibition at 5 mM
Aspergillus fumigatus
2.5.1.100
Zn2+
90% inhibition at 5 mM
Aspergillus fumigatus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.100
0.006
-
fumigaclavine A
pH 7.5, 30°C
Aspergillus fumigatus
2.5.1.100
0.013
-
dimethylallyl diphosphate
pH 7.5, 30°C
Aspergillus fumigatus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.5.1.100
soluble
-
Aspergillus fumigatus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.5.1.100
additional information
no significant effects by Mg2+, Ca2+, and EDTA at 5 mM
Aspergillus fumigatus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.100
110000
-
recombinant His6-tagged FgaPT1, gel filtration
Aspergillus fumigatus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
Aspergillus fumigatus
-
fumigaclavine C + diphosphate
-
-
?
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
Aspergillus fumigatus Af293
-
fumigaclavine C + diphosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.100
recombinant His6-tagged FgaPT1 from Escherichia coli strain BL21(DE3)pLysS to near homogeneity
Aspergillus fumigatus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
-
719293
Aspergillus fumigatus
fumigaclavine C + diphosphate
-
-
-
?
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus
719293
Aspergillus fumigatus
fumigaclavine C + diphosphate
-
-
-
?
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
-
719293
Aspergillus fumigatus Af293
fumigaclavine C + diphosphate
-
-
-
?
2.5.1.100
fumigaclavine A + dimethylallyl diphosphate
the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus
719293
Aspergillus fumigatus Af293
fumigaclavine C + diphosphate
-
-
-
?
2.5.1.100
additional information
FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A
719293
Aspergillus fumigatus
?
-
-
-
-
2.5.1.100
additional information
FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A
719293
Aspergillus fumigatus Af293
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.5.1.100
dimer
2 x 53000, recombinant His6-tagged FgaPT1, SDS-PAGE
Aspergillus fumigatus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.100
30
-
assay at
Aspergillus fumigatus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.100
0.8
-
dimethylallyl diphosphate
pH 7.5, 30°C
Aspergillus fumigatus
2.5.1.100
0.8
-
fumigaclavine A
pH 7.5, 30°C
Aspergillus fumigatus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.100
7.5
-
assay at
Aspergillus fumigatus
General Information
EC Number
General Information
Commentary
Organism
2.5.1.100
evolution
FgaPT1 shows very low sequence similarity to the recently identified prenyltransferase LtxC from cyanobacteria, which also catalyzes the reverse prenylation of an indole nucleus
Aspergillus fumigatus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.5.1.100
evolution
FgaPT1 shows very low sequence similarity to the recently identified prenyltransferase LtxC from cyanobacteria, which also catalyzes the reverse prenylation of an indole nucleus
Aspergillus fumigatus