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Literature summary extracted from

  • Carvajal, P.; Gibert, J.; Campos, N.; Lopera, O.; Barbera, E.; Torne, J.M.; Santos, M.
    Activity of maize transglutaminase overexpressed in Escherichia coli inclusion bodies: an alternative to protein refolding (2011), Biotechnol. Prog., 27, 232-240.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 expressed in inclusion bodies of Escherichia coli BL21(DE3) cells Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ required for activity Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 65000
-
 x * 65000, SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 N,N-dimethylcasein + putrescine Zea mays
-
 ?
-
 ?
2.3.2.13 N-carbobenzoxy-L-glutaminylglycine + NH2OH Zea mays specific high-affinity substrate L-glutamic acid-gamma-monohydroxamate + ?
-
 ?
2.3.2.13 thylakoid protein + putrescine Zea mays the incorporation of putrescine by the recombinant protein are 100fold greater using light-grown than dark-grown thylakoid protein extracts ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Zea mays
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.2.13 using 55 mM MES, 264 mM NaCl, 11 mM KCl, 0.055% (w/v) PEG 3350, 550 mM guanidine-HCl, 2.2 mM MgCl2, 2.2 mM CaCl2, and 440 mM sucrose Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 N,N-dimethylcasein + putrescine
-
 Zea mays ?
-
 ?
2.3.2.13 N-carbobenzoxy-L-glutaminylglycine + NH2OH specific high-affinity substrate Zea mays L-glutamic acid-gamma-monohydroxamate + ?
-
 ?
2.3.2.13 thylakoid protein + putrescine the incorporation of putrescine by the recombinant protein are 100fold greater using light-grown than dark-grown thylakoid protein extracts Zea mays ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 65000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 glutamine:amine gamma-glutamyl-transferase
-
 Zea mays
2.3.2.13 TGase
-
 Zea mays
2.3.2.13 transglutaminase
-
 Zea mays