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Literature summary extracted from

  • Nishiya, Y.; Tamura, N.; Tamura, T.
    Analysis of bacterial glucose dehydrogenase homologs from thermoacidophilic archaeon Thermoplasma acidophilum: finding and characterization of aldohexose dehydrogenase (2004), Biosci. Biotechnol. Biochem., 68, 2451-2456.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.121
-
 Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.121 Ag+
-
 Thermoplasma acidophilum
1.1.1.121 Cu2+
-
 Thermoplasma acidophilum
1.1.1.121 Hg2+
-
 Thermoplasma acidophilum
1.1.1.121 Ni2+
-
 Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.121 24.3
-
 2-deoxy-D-glucose pH 8.0, 37°C Thermoplasma acidophilum
1.1.1.121 25
-
 D-mannose pH 8.0, 37°C Thermoplasma acidophilum
1.1.1.121 208
-
 D-glucose pH 8.0, 37°C Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.121 30000
-
 x * 30000, SDS-PAGE Thermoplasma acidophilum
1.1.1.121 85000
-
 gel filtration Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.121 Thermoplasma acidophilum Q9HK51
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.121
-
 Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.121 4.5
-
 pH 8.0, 37°C, substrates: D-glucose + NAD+ Thermoplasma acidophilum
1.1.1.121 15
-
 pH 8.0, 37°C, substrates: D-xylose + NAD+ Thermoplasma acidophilum
1.1.1.121 24
-
 pH 8.0, 37°C, substrates: 2-deoxy-D-glucose + NAD+ Thermoplasma acidophilum
1.1.1.121 31
-
 pH 8.0, 37°C, substrates: D-mannose + NAD+ Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.121 2-deoxy-D-glucose + NAD+ relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+ Thermoplasma acidophilum 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.121 2-deoxy-D-glucose + NADP+ relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+ Thermoplasma acidophilum 2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.121 D-glucose + NAD+ activity is 23% compared to the activity with D-mannose and NAD+. No activity with beta-D-glucose + NADP+ Thermoplasma acidophilum D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.121 D-mannose + NAD+ relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+ Thermoplasma acidophilum D-mannono-1,5-lactone + NADH
-
 ?
1.1.1.121 D-mannose + NADP+ relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+ Thermoplasma acidophilum D-mannono-1,5-lactone + NADPH
-
 ?
1.1.1.121 D-xylose + NAD+ relative activity with D-xylose and NADP+ is less than 5% compared to the activity with D-xylose and NAD+ Thermoplasma acidophilum D-xylono-1,5-lactone + NADH + H+
-
 ?
1.1.1.121 D-xylose + NADP+ relative activity with D-xylose and NADP+ is less than 5% compared to the activity with D-xylose and NAD+ Thermoplasma acidophilum D-xylono-1,5-lactone + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.121 ? x * 30000, SDS-PAGE Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.121 aldohexose dehydrogenase
-
 Thermoplasma acidophilum
1.1.1.121 Ta0754
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.121 37
-
 assay at Thermoplasma acidophilum
1.1.1.121 70
-
-
 Thermoplasma acidophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.121 60
-
 stable up to, pH 7.5, 15 min Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.121 8
-
 assay at Thermoplasma acidophilum
1.1.1.121 10
-
-
 Thermoplasma acidophilum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.121 7 11 25°C, 16 h, no loss of activity Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.121 NAD+ relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+. Relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+ Thermoplasma acidophilum
1.1.1.121 NADP+ relative activity with D-mannose and NADP+ is 7% compared to the activity with D-mannose and NAD+. Relative activity with 2-deoxy-D-glucose and NADP+ is less than 5% compared to the activity with 2-deoxy-D-glucose and NAD+ Thermoplasma acidophilum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.121 Thermoplasma acidophilum isoelectric focusing
-
 5.2