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Literature summary extracted from

  • Osterman, I.A.; Sergiev, P.V.; Tsvetkov, P.O.; Makarov, A.A.; Bogdanov, A.A.; Dontsova, O.A.
    Methylated 23S rRNA nucleotide m2G1835 of Escherichia coli ribosome facilitates subunit association (2011), Biochimie, 93, 725-729.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.174 additional information generation of rlmG knock-out cells, ribosomes purified from the rlmG knockout strain do not have methyl group attached to nucleotide G1835 of the 23S rRNA Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.174 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.174 S-adenosyl-L-methionine + guanine1835 in 23S rRNA Escherichia coli m2G1835 is located at ribosomal subunits 50S and 30S interface S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.174 Escherichia coli
-
 gene rlmG
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.174 S-adenosyl-L-methionine + guanine1835 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
 ?
2.1.1.174 S-adenosyl-L-methionine + guanine1835 in 23S rRNA m2G1835 is located at ribosomal subunits 50S and 30S interface Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.174 N2-methyltransferase RlmG
-
 Escherichia coli
2.1.1.174 RlmG
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.174 S-adenosyl-L-methionine
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.174 malfunction absence of 23S rRNA nucleotide G1835 methylation does not influence the fidelity of translation or ribosome interaction with translation GTPases and decreases bacterial cell survival at osmotic and oxidative stress. Cells devoid of the rlmG gene are hypersensitive to osmotic and oxidative stress Escherichia coli
2.1.1.174 physiological function methylation of 23S rRNA nucleotide m2G1835 is important for association of ribosomal subunits. Methylation of G1835 provides a significant advantage for bacteria at osmotic and oxidative stress Escherichia coli