EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.7.11 | expression of mutant enzymes K125A and K173A in Escherichia coli | Sulfurisphaera tokodaii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.7.11 | K125A | the mutant enzyme shows a large increase in the Km-value for CoA and shows poor inactivation by 4-fluoro-7-nitrobenzofurazan, compared with K173A and wild type enzyme | Sulfurisphaera tokodaii |
1.2.7.11 | K173A | the mutant enzyme shows a large increase in the Km-value for CoA and shows poor inactivation by 4-fluoro-7-nitrobenzofurazan, compared with K173A and wild type enzyme | Sulfurisphaera tokodaii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.11 | 4-fluoro-7-nitrobenzofurazan | 0.001 mM inhibitor with 0.01 mM enzyme decreases the activity by about 20% and 0.003 mM inhibitor with 0.01 mM enzyme decreases the activity by around 50%. Inactivation is prevented by CoA | Sulfurisphaera tokodaii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.11 | 0.05 | - |
CoA | pH 8.5, 80°C, mutant enzyme K173A | Sulfurisphaera tokodaii | |
1.2.7.11 | 0.074 | - |
CoA | pH 8.5, 80°C, wild-type enzyme | Sulfurisphaera tokodaii | |
1.2.7.11 | 1.17 | - |
CoA | pH 8.5, 80°C, mutant enzyme K125A | Sulfurisphaera tokodaii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.11 | Sulfurisphaera tokodaii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.11 | - |
Sulfurisphaera tokodaii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.11 | pyruvate + CoA + 2 oxidized ferredoxin | Lys125 in subunit b is the critical residue that interacts with CoA | Sulfurisphaera tokodaii | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.11 | StOFOR | - |
Sulfurisphaera tokodaii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.11 | 80 | - |
assay at | Sulfurisphaera tokodaii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.11 | 8.5 | - |
assay at | Sulfurisphaera tokodaii |