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Literature summary extracted from

  • Meshalkina, L.E.; Solovjeva, O.N.; Khodak, Y.A.; Drutsa, V.L.; Kochetov, G.A.
    Isolation and properties of human transketolase (2010), Biochemistry (Moscow), 75, 873-880.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.1 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 additional information in vitro induction of the expression by arabinose decreases the amount of recombinant enzyme isolated and lowers its specific activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.011
-
 D-xylulose 5-phosphate pH 7.6, 22°C Homo sapiens
2.2.1.1 0.063
-
 D-ribose 5-phosphate pH 7.6, 22°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 additional information presence of magnesium ions, alone or in combination with thiamine diphosphate, does not affect the activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 67900
-
 x * 68000, SDS-PAGE, x * 67900, calculated Homo sapiens
2.2.1.1 68000
-
 x * 68000, SDS-PAGE, x * 67900, calculated Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 recombinant protein Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.1 3.64
-
 pH 7.6, 22°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.2.1.1 -18°C, 50 mM glycylglycine buffer, pH 7.6, no activity loss throughout several months Homo sapiens
2.2.1.1 4°C, 50 mM glycylglycine buffer, pH 7.6, stable for several days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 2 D-xylulose 5-phosphate reversible single substrate reaction, about 6% of the double-substrate reaction rate Homo sapiens glyceraldehyde 3-phosphate + glycolaldehyde
-
 ?
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Homo sapiens sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.1 ? x * 68000, SDS-PAGE, x * 67900, calculated Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate Km value is 0.074 mM Homo sapiens