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Literature summary extracted from

  • Knirel, Y.; Senchenkova, S.; Kocharova, N.; Shashkov, A.; Helbig, J.; Zaehringer, U.
    Identification of a homopolymer of 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-nonulosonic acid in the lipopolysaccharides of Legionella pneumophila non-1 serogroups (2001), Biochemistry, 66, 1035-1041.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.101 gene lpg 0752, cloning and expression of NeuB results in insoluble protein which possesses very low levels of N,N'-diacetyllegionaminate synthase activity Legionella pneumophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.101 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O Legionella pneumophila
-
 N,N'-diacetyllegionaminate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.101 Legionella pneumophila
-
 serogroups 2-14, and strains Lansing 3 and 16453-92 from another serogroup, gene lpg 0752 or neuB homologue
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.101 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
 Legionella pneumophila N,N'-diacetyllegionaminate + phosphate
-
 ?
2.5.1.101 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
 Legionella pneumophila N,N'-diacetyllegionaminate + phosphate i.e. Leg5Ac7Ac ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.101 NeuB
-
 Legionella pneumophila
2.5.1.101 nonulosonic acid synthase
-
 Legionella pneumophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.101 37
-
 assay at Legionella pneumophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.101 7.5
-
 assay at Legionella pneumophila

General Information

EC Number General Information Comment Organism
2.5.1.101 metabolism the enzyme is involved in the the Leg pathway, which is essential for the biosynthesis of CMP-N,N'-diacetyllegionaminate, that acts as a donor substrate with a selection of bacterial and mammalian sialyltransferases Legionella pneumophila
2.5.1.101 additional information NeuB can be substituted by NanA from Escherichia coli, a sialic acid aldolase that catalyzes the condensation of 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose with pyruvate resulting in N,N'-diacetyllegionaminate formation, ability of sialyltransferases to utilize the CMP-N,N'-diacetyllegionaminate donor, overview Legionella pneumophila