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Literature summary extracted from

  • Borgaro, J.; Chang, A.; MacHutta, C.; Zhang, X.; Tonge, P.
    Substrate recognition by beta-ketoacyl-ACP synthases (2011), Biochemistry, 50, 10678-10686.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 His-tagged protein expressed in Escherichia coli pLysS Escherichia coli
2.3.1.41 His-tagged proteinsexpressed in Mycobacterium smegmatis mc2155 Mycobacterium tuberculosis
2.3.1.179 His-tagged protein expressed in Escherichia coli pLysS Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.41 C171Q mimics the acyl enzyme intermediate Mycobacterium tuberculosis
2.3.1.41 K151Q conserved between FabB, FabF and KasA, proposed to be important for ACP recognition Escherichia coli
2.3.1.41 K63Q conserved between FabB, FabF and KasA, proposed to be important for ACP recognition Escherichia coli
2.3.1.41 R62Q conserved between FabB, FabF and KasA, proposed to be important for ACP recognition Escherichia coli
2.3.1.41 R66Q conserved between FabB, FabF and KasA, proposed to be important for ACP recognition Escherichia coli
2.3.1.179 R206G R206 impairs the binding of CoA Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 palmitoyl-CoA substrate inhibition above 0.003 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.41 0.0004
-
 palmitoyl-CoA acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.0032
-
 lauroyl-ACP wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.0039
-
 lauroyl-ACP wild type protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.0047
-
 malonyl-phosphopantetheine-8-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.0051
-
 malonyl-phosphopantetheine-16-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.0058
-
 malonyl-ACP donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.0067
-
 lauroyl-ACP K151Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.0086
-
 malonyl-phosphopantetheine-14-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.009
-
 malonyl-CoA donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.0115
-
 malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.0142
-
 malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.0158
-
 malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.029
-
 malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.05
-
 lauroyl-ACP R62Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.056
-
 lauroyl-ACP R66Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.0586
-
 lauroyl-CoA wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.06
-
 lauroyl-ACP K63Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.153
-
 malonyl-CoA wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.0061
-
 malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.0062
-
 malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.0082
-
 malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.0237
-
 malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.0283
-
 malonyl-CoA R206G mutant protein, donor lauroyl-CoA, 0.1 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.0537
-
 lauroyl-CoA wild type protein, acceptor malonyl-CoA, 0.5 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.51
-
 malonyl-CoA wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.59
-
 malonyl-phosphopantetheine wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Escherichia coli
-
-
-
2.3.1.41 Mycobacterium tuberculosis
-
-
-
2.3.1.179 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 immobilized metal ion affinity chromatography (Ni2+) Escherichia coli
2.3.1.41 immobilized metal ion affinity chromatography (Ni2+) Mycobacterium tuberculosis
2.3.1.179 immobilized metal ion affinity chromatography (Ni2+) Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 malonyl-ACP + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
2.3.1.41 malonyl-ACP + lauroyl-CoA
-
 Escherichia coli ?
-
 ?
2.3.1.41 malonyl-ACP + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.41 malonyl-ACP + palmitoyl-CoA
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.41 malonyl-CoA + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
2.3.1.41 malonyl-CoA + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.41 malonyl-phosphopantetheine-14-mer + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
2.3.1.41 malonyl-phosphopantetheine-14-mer + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.41 malonyl-phosphopantetheine-16-mer + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
2.3.1.41 malonyl-phosphopantetheine-16-mer + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.41 malonyl-phosphopantetheine-8-mer + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
2.3.1.41 malonyl-phosphopantetheine-8-mer + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.179 malonyl-ACP + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
2.3.1.179 malonyl-CoA + lauroyl-CoA
-
 Escherichia coli ?
-
 ?
2.3.1.179 malonyl-phosphopantetheine + lauroyl-CoA
-
 Escherichia coli ?
-
 ?
2.3.1.179 malonyl-phosphopantetheine-14-mer + lauroyl-CoA
-
 Escherichia coli ?
-
 ?
2.3.1.179 malonyl-phosphopantetheine-16-mer + lauroyl-CoA
-
 Escherichia coli ?
-
 ?
2.3.1.179 malonyl-phosphopantetheine-8-mer + lauroyl-CoA
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.41 beta-ketoacyl-ACP synthase I
-
 Escherichia coli
2.3.1.41 beta-ketoacyl-ACP synthase I
-
 Mycobacterium tuberculosis
2.3.1.41 FabB
-
 Escherichia coli
2.3.1.41 KasA
-
 Mycobacterium tuberculosis
2.3.1.179 beta-ketoacyl-ACP synthase II
-
 Escherichia coli
2.3.1.179 FabF
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.41 0.011
-
 malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.018
-
 malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.025
-
 lauroyl-ACP K151Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.033
-
 lauroyl-ACP wild type protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.04
-
 malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.053
-
 lauroyl-ACP R66Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.057
-
 lauroyl-ACP wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.073
-
 lauroyl-CoA wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.077
-
 lauroyl-ACP R62Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.08
-
 palmitoyl-CoA acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.083
-
 lauroyl-ACP K63Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.11
-
 malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.12
-
 malonyl-CoA wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.3
-
 malonyl-phosphopantetheine-16-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.35
-
 malonyl-CoA donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.35
-
 malonyl-phosphopantetheine-8-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.38
-
 malonyl-phosphopantetheine-14-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 0.47
-
 malonyl-ACP donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.179 0.018
-
 malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.022
-
 malonyl-CoA R206G mutant protein, donor lauroyl-CoA, 0.1 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.022
-
 malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.022
-
 malonyl-phosphopantetheine wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.023
-
 malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.029
-
 malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.042
-
 malonyl-CoA wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.047
-
 lauroyl-CoA wild type protein, acceptor malonyl-CoA, 0.5 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.41 0.78
-
 malonyl-CoA wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 0.83
-
 lauroyl-ACP R66Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 1.2
-
 lauroyl-ACP K63Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 1.27
-
 lauroyl-CoA wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 1.3
-
 malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 1.5
-
 lauroyl-ACP R62Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 3
-
 malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 3.7
-
 lauroyl-ACP K151Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 3.8
-
 malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 8.3
-
 lauroyl-ACP wild type protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.41 10
-
 malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 18.3
-
 lauroyl-ACP wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.41 38.3
-
 malonyl-CoA donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 48.3
-
 malonyl-phosphopantetheine-14-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 60
-
 malonyl-phosphopantetheine-16-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 75
-
 malonyl-phosphopantetheine-8-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.41 86.7
-
 malonyl-ACP donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
2.3.1.179 0.037
-
 malonyl-phosphopantetheine wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.083
-
 malonyl-CoA wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.083
-
 malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 0.83
-
 malonyl-CoA R206G mutant protein, donor lauroyl-CoA, 0.1 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 1
-
 lauroyl-CoA wild type protein, acceptor malonyl-CoA, 0.5 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 3.8
-
 malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
2.3.1.179 3.8
-
 malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli
2.3.1.179 4.2
-
 malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-CoA, 0.075 mM lauroyl-CoA, pH 6.5, 25°C Escherichia coli