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Literature summary extracted from

  • Zhu, X.; Kim, J.; Su, X.; Lin, H.
    Reconstitution of diphthine synthase activity in vitro (2010), Biochemistry, 49, 9649-9657.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.98 expression in Escherichia coli BL21 (DE3) Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.98 30000
-
 x * 30000, SDS-PAGE Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.98 3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] Pyrococcus horikoshii the enzyme acts on His600 of elongation factor 2. It is involved in diphthamide biosynthesis 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2]
-
 ?
2.1.1.98 3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] Pyrococcus horikoshii DSM 12428 the enzyme acts on His600 of elongation factor 2. It is involved in diphthamide biosynthesis 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.98 Pyrococcus horikoshii O58456
-
-
2.1.1.98 Pyrococcus horikoshii DSM 12428 O58456
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.98 3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] the enzyme acts on His600 of elongation factor 2. It is involved in diphthamide biosynthesis Pyrococcus horikoshii 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2]
-
 ?
2.1.1.98 3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group Pyrococcus horikoshii 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2]
-
 ?
2.1.1.98 3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] the enzyme acts on His600 of elongation factor 2. It is involved in diphthamide biosynthesis Pyrococcus horikoshii DSM 12428 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2]
-
 ?
2.1.1.98 3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group Pyrococcus horikoshii DSM 12428 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2]
-
 ?
2.1.1.98 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group Pyrococcus horikoshii S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2]
-
 ?
2.1.1.98 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group Pyrococcus horikoshii DSM 12428 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2]
-
 ?
2.1.1.98 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group Pyrococcus horikoshii S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2]
-
 ?
2.1.1.98 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group Pyrococcus horikoshii DSM 12428 S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2]
-
 ?
2.1.1.98 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group Pyrococcus horikoshii S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2]
-
 ?
2.1.1.98 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group Pyrococcus horikoshii DSM 12428 S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.98 ? x * 30000, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
2.1.1.98 Dph5
-
 Pyrococcus horikoshii

General Information

EC Number General Information Comment Organism
2.1.1.98 physiological function the enzyme is involved in diphthamide biosynthesis Pyrococcus horikoshii