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Literature summary extracted from

  • French, J.B.; Cen, Y.; Sauve, A.A.; Ealick, S.E.
    High-resolution crystal structures of Streptococcus pneumoniae nicotinamidase with trapped intermediates provide insights into the catalytic mechanism and inhibition by aldehydes (2010), Biochemistry, 49, 8803-8812.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.19 expressed in Escherichia coli Rosetta 2(DE3) pLysS cells Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.19 a trapped nicotinoyl-thioester complexed with wild type enzyme and C136S mutant in complex with nicotinamide, hanging drop vapor diffusion method, using 18-22% (w/v) polyethylene glycol 3350, 0.2-0.3 M NaCl and 0.2 M sodium malonate, pH 6.3, at 18°C Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.19 C136S inactive Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.19 5-methoxynicotinaldehyde competitive inhibitor Streptococcus pneumoniae
3.5.1.19 nicotinaldehyde competitive inhibitor Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.19 Co2+ addition of Co2+ after metal removement results in 13% activity Streptococcus pneumoniae
3.5.1.19 Fe2+ addition of Fe2+ after metal removement results in 13% activity Streptococcus pneumoniae
3.5.1.19 Mn2+ addition of Mn2+ after metal removement results in 27% activity Streptococcus pneumoniae
3.5.1.19 additional information addition of Fe3+ or Ni2+ after metal removement results in no detectable activity Streptococcus pneumoniae
3.5.1.19 Zn2+ contains zinc, highest level of activity in the presence of Zn2+ Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.19 21400
-
4 * 21400, calculated from amino acid sequence Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Streptococcus pneumoniae
-
nicotinate + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Streptococcus pneumoniae A0A0H2UR34
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.19 Ni-NTA resin column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O
-
Streptococcus pneumoniae nicotinate + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.19 homotetramer 4 * 21400, calculated from amino acid sequence Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
3.5.1.19 NIC
-
Streptococcus pneumoniae