Literature summary extracted from
French, J.B.; Cen, Y.; Sauve, A.A.; Ealick, S.E.
High-resolution crystal structures of Streptococcus pneumoniae nicotinamidase with trapped intermediates provide insights into the catalytic mechanism and inhibition by aldehydes (2010), Biochemistry, 49, 8803-8812.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.5.1.19 |
expressed in Escherichia coli Rosetta 2(DE3) pLysS cells |
Streptococcus pneumoniae |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.5.1.19 |
a trapped nicotinoyl-thioester complexed with wild type enzyme and C136S mutant in complex with nicotinamide, hanging drop vapor diffusion method, using 18-22% (w/v) polyethylene glycol 3350, 0.2-0.3 M NaCl and 0.2 M sodium malonate, pH 6.3, at 18°C |
Streptococcus pneumoniae |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.5.1.19 |
C136S |
inactive |
Streptococcus pneumoniae |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.5.1.19 |
5-methoxynicotinaldehyde |
competitive inhibitor |
Streptococcus pneumoniae |
|
3.5.1.19 |
nicotinaldehyde |
competitive inhibitor |
Streptococcus pneumoniae |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.5.1.19 |
Co2+ |
addition of Co2+ after metal removement results in 13% activity |
Streptococcus pneumoniae |
|
3.5.1.19 |
Fe2+ |
addition of Fe2+ after metal removement results in 13% activity |
Streptococcus pneumoniae |
|
3.5.1.19 |
Mn2+ |
addition of Mn2+ after metal removement results in 27% activity |
Streptococcus pneumoniae |
|
3.5.1.19 |
additional information |
addition of Fe3+ or Ni2+ after metal removement results in no detectable activity |
Streptococcus pneumoniae |
|
3.5.1.19 |
Zn2+ |
contains zinc, highest level of activity in the presence of Zn2+ |
Streptococcus pneumoniae |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.5.1.19 |
21400 |
- |
4 * 21400, calculated from amino acid sequence |
Streptococcus pneumoniae |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.5.1.19 |
nicotinamide + H2O |
Streptococcus pneumoniae |
- |
nicotinate + NH3 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.5.1.19 |
Streptococcus pneumoniae |
A0A0H2UR34 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.5.1.19 |
Ni-NTA resin column chromatography |
Streptococcus pneumoniae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.5.1.19 |
nicotinamide + H2O |
- |
Streptococcus pneumoniae |
nicotinate + NH3 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.5.1.19 |
homotetramer |
4 * 21400, calculated from amino acid sequence |
Streptococcus pneumoniae |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.5.1.19 |
NIC |
- |
Streptococcus pneumoniae |