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Literature summary extracted from
- Characterization of nicotinamidases: steady state kinetic parameters, classwide inhibition by nicotinaldehydes, and catalytic mechanism (2010), Biochemistry, 49, 10421-10439.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.19 | - |
Saccharomyces cerevisiae |
| 3.5.1.19 | - |
Streptococcus pneumoniae |
| 3.5.1.19 | - |
Caenorhabditis elegans |
| 3.5.1.19 | - |
Plasmodium falciparum |
| 3.5.1.19 | - |
Borreliella burgdorferi |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | C136A | the mutant is unable to catalyze nicotinamide hydrolysis | Streptococcus pneumoniae |
| 3.5.1.19 | C136S | the mutant is unable to catalyze nicotinamide hydrolysis | Streptococcus pneumoniae |
| 3.5.1.19 | K103A | the mutant has only 0.15% of the catalytic activity of the native enzyme | Streptococcus pneumoniae |
| 3.5.1.19 | R97A | the mutant is a robust nicotinamidase and is nearly as good as the native enzyme | Streptococcus pneumoniae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.19 | 2-chloropyridine-3-carbaldehyde | - |
Borreliella burgdorferi |  |
| 3.5.1.19 | 2-chloropyridine-3-carbaldehyde | - |
Saccharomyces cerevisiae | |
| 3.5.1.19 | 3-acetylpyridine | - |
Borreliella burgdorferi | |
| 3.5.1.19 | 3-acetylpyridine | - |
Plasmodium falciparum | |
| 3.5.1.19 | 3-acetylpyridine | - |
Saccharomyces cerevisiae | |
| 3.5.1.19 | 3-acetylpyridine | - |
Streptococcus pneumoniae | |
| 3.5.1.19 | 3-Cyanopyridine | - |
Borreliella burgdorferi | |
| 3.5.1.19 | 3-Cyanopyridine | - |
Plasmodium falciparum | |
| 3.5.1.19 | 3-Cyanopyridine | - |
Saccharomyces cerevisiae | |
| 3.5.1.19 | 3-Cyanopyridine | - |
Streptococcus pneumoniae | |
| 3.5.1.19 | 3-hydroxypyridine | - |
Streptococcus pneumoniae | |
| 3.5.1.19 | 4-methoxylnicotinaldehyde | weak inhibitor | Borreliella burgdorferi | |
| 3.5.1.19 | 4-methoxylnicotinaldehyde | weak inhibitor | Caenorhabditis elegans | |
| 3.5.1.19 | 4-methoxylnicotinaldehyde | weak inhibitor | Plasmodium falciparum | |
| 3.5.1.19 | 4-methoxylnicotinaldehyde | weak inhibitor | Saccharomyces cerevisiae | |
| 3.5.1.19 | 4-methoxylnicotinaldehyde | weak inhibitor | Streptococcus pneumoniae | |
| 3.5.1.19 | 5-bromopyridine-3-carbaldehyde | potent inhibitor | Plasmodium falciparum | |
| 3.5.1.19 | 5-bromopyridine-3-carbaldehyde | potent inhibitor | Saccharomyces cerevisiae | |
| 3.5.1.19 | 5-methoxypyridine-3-carbaldehyde | potent inhibitor | Borreliella burgdorferi | |
| 3.5.1.19 | 5-methoxypyridine-3-carbaldehyde | potent inhibitor | Caenorhabditis elegans | |
| 3.5.1.19 | 5-methoxypyridine-3-carbaldehyde | potent inhibitor | Plasmodium falciparum | |
| 3.5.1.19 | 5-methoxypyridine-3-carbaldehyde | potent inhibitor | Saccharomyces cerevisiae | |
| 3.5.1.19 | 5-methoxypyridine-3-carbaldehyde | potent inhibitor | Streptococcus pneumoniae | |
| 3.5.1.19 | 5-methylnicotinaldehyde | - |
Borreliella burgdorferi | |
| 3.5.1.19 | 5-methylnicotinaldehyde | - |
Caenorhabditis elegans | |
| 3.5.1.19 | 5-methylnicotinaldehyde | - |
Plasmodium falciparum | |
| 3.5.1.19 | 5-methylnicotinaldehyde | - |
Saccharomyces cerevisiae | |
| 3.5.1.19 | 5-methylnicotinaldehyde | - |
Streptococcus pneumoniae | |
| 3.5.1.19 | 5-methylpyridine-3-carbaldehyde | potent inhibitor | Borreliella burgdorferi | |
| 3.5.1.19 | 5-methylpyridine-3-carbaldehyde | potent inhibitor | Plasmodium falciparum | |
| 3.5.1.19 | 5-methylpyridine-3-carbaldehyde | potent inhibitor | Saccharomyces cerevisiae | |
| 3.5.1.19 | 6-fluoropyridine-3-carbaldehyde | - |
Borreliella burgdorferi | |
| 3.5.1.19 | 6-fluoropyridine-3-carbaldehyde | - |
Plasmodium falciparum | |
| 3.5.1.19 | additional information | not inhibited by 3-hydroxypyridine | Borreliella burgdorferi | |
| 3.5.1.19 | additional information | not inhibited by nicotinic acid | Caenorhabditis elegans | |
| 3.5.1.19 | additional information | not inhibited by 3-hydroxypyridine and 2-chloropyridine-3-carbaldehyde | Plasmodium falciparum | |
| 3.5.1.19 | additional information | not inhibited by 3-hydroxypyridine and 6-fluoropyridine-3-carbaldehyde | Saccharomyces cerevisiae | |
| 3.5.1.19 | nicotinaldehyde | potent competitive inhibitor | Borreliella burgdorferi | |
| 3.5.1.19 | nicotinaldehyde | potent competitive inhibitor | Caenorhabditis elegans | |
| 3.5.1.19 | nicotinaldehyde | potent competitive inhibitor | Plasmodium falciparum | |
| 3.5.1.19 | nicotinaldehyde | potent competitive inhibitor | Saccharomyces cerevisiae | |
| 3.5.1.19 | nicotinaldehyde | potent competitive inhibitor | Streptococcus pneumoniae | |
| 3.5.1.19 | nicotinic acid | - |
Borreliella burgdorferi | |
| 3.5.1.19 | nicotinic acid | - |
Plasmodium falciparum | |
| 3.5.1.19 | nicotinic acid | - |
Saccharomyces cerevisiae | |
| 3.5.1.19 | nicotinic acid | - |
Streptococcus pneumoniae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.19 | 0.0027 | - |
nicotinamide | wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C | Streptococcus pneumoniae | |
| 3.5.1.19 | 0.003 | - |
nicotinamide | wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 0.0106 | - |
nicotinamide | wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C | Saccharomyces cerevisiae | |
| 3.5.1.19 | 0.0367 | - |
nicotinamide | wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C | Borreliella burgdorferi | |
| 3.5.1.19 | 0.076 | - |
nicotinamide | wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C | Caenorhabditis elegans | |
| 3.5.1.19 | 0.113 | - |
nicotinamide | wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C | Caenorhabditis elegans | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.19 | 4-methoxypyridine-3-carboxamide + H2O | Plasmodium falciparum | 12% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 4-methoxypyridine-3-carboxamide + H2O | Borreliella burgdorferi | 20% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 4-methoxypyridine-3-carboxamide + H2O | Saccharomyces cerevisiae | 5% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 4-methoxypyridine-3-carboxamide + H2O | Streptococcus pneumoniae | 6.5% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 5-methoxypyridine-3-carboxamide + H2O | Streptococcus pneumoniae | 137% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 5-methoxypyridine-3-carboxamide + H2O | Saccharomyces cerevisiae | 206% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 5-methoxypyridine-3-carboxamide + H2O | Borreliella burgdorferi | 221% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 5-methoxypyridine-3-carboxamide + H2O | Plasmodium falciparum | 646% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 5-methylpyridine-3-carboxamide + H2O | Streptococcus pneumoniae | 163% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 5-methylpyridine-3-carboxamide + H2O | Plasmodium falciparum | 330% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 5-methylpyridine-3-carboxamide + H2O | Borreliella burgdorferi | 51% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 5-methylpyridine-3-carboxamide + H2O | Saccharomyces cerevisiae | 80% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 6-aminopyridine-3-carboxamide + H2O | Saccharomyces cerevisiae | 20% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 6-aminopyridine-3-carboxamide + H2O | Streptococcus pneumoniae | 36% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 6-aminopyridine-3-carboxamide + H2O | Plasmodium falciparum | 45% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 6-chloropyridine-3-carboxamide + H2O | Streptococcus pneumoniae | 1.8% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 6-chloropyridine-3-carboxamide + H2O | Saccharomyces cerevisiae | 17% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 6-methylpyridine-3-carboxamide + H2O | Streptococcus pneumoniae | 11% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 6-methylpyridine-3-carboxamide + H2O | Plasmodium falciparum | 40% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | 6-methylpyridine-3-carboxamide + H2O | Borreliella burgdorferi | 45% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | ethyl pyridine-3-carboxylate + H2O | Streptococcus pneumoniae | 0.86% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | ethyl pyridine-3-carboxylate + H2O | Plasmodium falciparum | 2.3% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | ethyl pyridine-3-carboxylate + H2O | Saccharomyces cerevisiae | 4.4% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | methyl pyridine-3-carboxylate + H2O | Saccharomyces cerevisiae | 15% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | methyl pyridine-3-carboxylate + H2O | Streptococcus pneumoniae | 3.5% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | methyl pyridine-3-carboxylate + H2O | Plasmodium falciparum | 6500% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | additional information | Saccharomyces cerevisiae | nicotinamidase cannot hydrolyze 1-methyl isonicotinamide | ? | - |
? | |
| 3.5.1.19 | additional information | Plasmodium falciparum | nicotinamidase cannot hydrolyze 1-methyl isonicotinamide | ? | - |
? | |
| 3.5.1.19 | additional information | Borreliella burgdorferi | nicotinamidase cannot hydrolyze 1-methyl isonicotinamide | ? | - |
? | |
| 3.5.1.19 | additional information | Streptococcus pneumoniae | nicotinamidase cannot hydrolyze 1-methylisonicotinamide | ? | - |
? | |
| 3.5.1.19 | additional information | Caenorhabditis elegans | nicotinamidase cannot hydrolyze 1-methylisonicotinamide | ? | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | Saccharomyces cerevisiae | 100% activity | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | Streptococcus pneumoniae | 100% activity | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | Caenorhabditis elegans | 100% activity | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | Plasmodium falciparum | 100% activity | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | Borreliella burgdorferi | 100% activity | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | phenyl pyridine-3-carboxylate + H2O | Plasmodium falciparum | 13% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | phenyl pyridine-3-carboxylate + H2O | Saccharomyces cerevisiae | 3.1% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | phenyl pyridine-3-carboxylate + H2O | Streptococcus pneumoniae | 3.1% activity compared to nicotinamide | ? | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | Saccharomyces cerevisiae | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | Streptococcus pneumoniae | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | Caenorhabditis elegans | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | Plasmodium falciparum | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | Borreliella burgdorferi | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyridine-3-carbothioamide + H2O | Saccharomyces cerevisiae | 44% activity compared to nicotinamide | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.19 | Borreliella burgdorferi | - |
- |
- |
| 3.5.1.19 | Caenorhabditis elegans | - |
- |
- |
| 3.5.1.19 | Plasmodium falciparum | - |
- |
- |
| 3.5.1.19 | Saccharomyces cerevisiae | - |
- |
- |
| 3.5.1.19 | Streptococcus pneumoniae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.19 | - |
Saccharomyces cerevisiae |
| 3.5.1.19 | - |
Streptococcus pneumoniae |
| 3.5.1.19 | - |
Caenorhabditis elegans |
| 3.5.1.19 | - |
Plasmodium falciparum |
| 3.5.1.19 | - |
Borreliella burgdorferi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.19 | 4-methoxypyridine-3-carboxamide + H2O | 12% activity compared to nicotinamide | Plasmodium falciparum | ? | - |
? | |
| 3.5.1.19 | 4-methoxypyridine-3-carboxamide + H2O | 20% activity compared to nicotinamide | Borreliella burgdorferi | ? | - |
? | |
| 3.5.1.19 | 4-methoxypyridine-3-carboxamide + H2O | 5% activity compared to nicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
| 3.5.1.19 | 4-methoxypyridine-3-carboxamide + H2O | 6.5% activity compared to nicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | 5-methoxypyridine-3-carboxamide + H2O | 137% activity compared to nicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | 5-methoxypyridine-3-carboxamide + H2O | 206% activity compared to nicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
| 3.5.1.19 | 5-methoxypyridine-3-carboxamide + H2O | 221% activity compared to nicotinamide | Borreliella burgdorferi | ? | - |
? | |
| 3.5.1.19 | 5-methoxypyridine-3-carboxamide + H2O | 646% activity compared to nicotinamide | Plasmodium falciparum | ? | - |
? | |
| 3.5.1.19 | 5-methylpyridine-3-carboxamide + H2O | 163% activity compared to nicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | 5-methylpyridine-3-carboxamide + H2O | 330% activity compared to nicotinamide | Plasmodium falciparum | ? | - |
? | |
| 3.5.1.19 | 5-methylpyridine-3-carboxamide + H2O | 51% activity compared to nicotinamide | Borreliella burgdorferi | ? | - |
? | |
| 3.5.1.19 | 5-methylpyridine-3-carboxamide + H2O | 80% activity compared to nicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
| 3.5.1.19 | 6-aminopyridine-3-carboxamide + H2O | 20% activity compared to nicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
| 3.5.1.19 | 6-aminopyridine-3-carboxamide + H2O | 36% activity compared to nicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | 6-aminopyridine-3-carboxamide + H2O | 45% activity compared to nicotinamide | Plasmodium falciparum | ? | - |
? | |
| 3.5.1.19 | 6-chloropyridine-3-carboxamide + H2O | 1.8% activity compared to nicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | 6-chloropyridine-3-carboxamide + H2O | 17% activity compared to nicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
| 3.5.1.19 | 6-methylpyridine-3-carboxamide + H2O | 11% activity compared to nicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | 6-methylpyridine-3-carboxamide + H2O | 40% activity compared to nicotinamide | Plasmodium falciparum | ? | - |
? | |
| 3.5.1.19 | 6-methylpyridine-3-carboxamide + H2O | 45% activity compared to nicotinamide | Borreliella burgdorferi | ? | - |
? | |
| 3.5.1.19 | ethyl pyridine-3-carboxylate + H2O | 0.86% activity compared to nicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | ethyl pyridine-3-carboxylate + H2O | 2.3% activity compared to nicotinamide | Plasmodium falciparum | ? | - |
? | |
| 3.5.1.19 | ethyl pyridine-3-carboxylate + H2O | 4.4% activity compared to nicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
| 3.5.1.19 | methyl pyridine-3-carboxylate + H2O | 15% activity compared to nicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
| 3.5.1.19 | methyl pyridine-3-carboxylate + H2O | 3.5% activity compared to nicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | methyl pyridine-3-carboxylate + H2O | 6500% activity compared to nicotinamide | Plasmodium falciparum | ? | - |
? | |
| 3.5.1.19 | additional information | nicotinamidase cannot hydrolyze 1-methyl isonicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
| 3.5.1.19 | additional information | nicotinamidase cannot hydrolyze 1-methyl isonicotinamide | Plasmodium falciparum | ? | - |
? | |
| 3.5.1.19 | additional information | nicotinamidase cannot hydrolyze 1-methyl isonicotinamide | Borreliella burgdorferi | ? | - |
? | |
| 3.5.1.19 | additional information | nicotinamidase cannot hydrolyze 1-methylisonicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | additional information | nicotinamidase cannot hydrolyze 1-methylisonicotinamide | Caenorhabditis elegans | ? | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | 100% activity | Saccharomyces cerevisiae | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | 100% activity | Streptococcus pneumoniae | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | 100% activity | Caenorhabditis elegans | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | 100% activity | Plasmodium falciparum | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | nicotinamide + H2O | 100% activity | Borreliella burgdorferi | nicotinate + NH3 | - |
? | |
| 3.5.1.19 | phenyl pyridine-3-carboxylate + H2O | 13% activity compared to nicotinamide | Plasmodium falciparum | ? | - |
? | |
| 3.5.1.19 | phenyl pyridine-3-carboxylate + H2O | 3.1% activity compared to nicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
| 3.5.1.19 | phenyl pyridine-3-carboxylate + H2O | 3.1% activity compared to nicotinamide | Streptococcus pneumoniae | ? | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | - |
Saccharomyces cerevisiae | pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | - |
Streptococcus pneumoniae | pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | - |
Caenorhabditis elegans | pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | - |
Plasmodium falciparum | pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | - |
Borreliella burgdorferi | pyrazine-2-carboxylic acid + NH3 | - |
? | |
| 3.5.1.19 | pyridine-3-carbothioamide + H2O | 44% activity compared to nicotinamide | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | NIC | - |
Streptococcus pneumoniae |
| 3.5.1.19 | NIC | - |
Plasmodium falciparum |
| 3.5.1.19 | NIC | - |
Borreliella burgdorferi |
| 3.5.1.19 | Pnc1 | - |
Saccharomyces cerevisiae |
| 3.5.1.19 | Pnc1 | isoform | Caenorhabditis elegans |
| 3.5.1.19 | PNC2 | isoform | Caenorhabditis elegans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.19 | 0.003 | - |
nicotinamide | wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C | Borreliella burgdorferi | |
| 3.5.1.19 | 0.28 | - |
nicotinamide | wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C | Caenorhabditis elegans | |
| 3.5.1.19 | 0.3 | - |
nicotinamide | wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 2.1 | - |
nicotinamide | wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C | Streptococcus pneumoniae | |
| 3.5.1.19 | 3.1 | - |
nicotinamide | wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C | Caenorhabditis elegans | |
| 3.5.1.19 | 3.8 | - |
nicotinamide | wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C | Saccharomyces cerevisiae | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.19 | 0.000011 | - |
nicotinaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Streptococcus pneumoniae | |
| 3.5.1.19 | 0.000022 | - |
nicotinaldehyde | isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C | Caenorhabditis elegans | |
| 3.5.1.19 | 0.000023 | - |
5-methylnicotinaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 0.000023 | - |
5-methylpyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 0.000034 | - |
nicotinaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 0.000039 | - |
5-methoxypyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 0.000056 | - |
5-methoxypyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Streptococcus pneumoniae | |
| 3.5.1.19 | 0.00011 | - |
nicotinaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Borreliella burgdorferi | |
| 3.5.1.19 | 0.00011 | - |
nicotinaldehyde | isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C | Caenorhabditis elegans | |
| 3.5.1.19 | 0.00014 | - |
5-methoxypyridine-3-carbaldehyde | isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C | Caenorhabditis elegans | |
| 3.5.1.19 | 0.00019 | - |
5-methylpyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Borreliella burgdorferi | |
| 3.5.1.19 | 0.00031 | - |
5-methoxypyridine-3-carbaldehyde | isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C | Caenorhabditis elegans | |
| 3.5.1.19 | 0.00044 | - |
4-methoxylnicotinaldehyde | isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C | Caenorhabditis elegans | |
| 3.5.1.19 | 0.00059 | - |
5-bromopyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 0.00065 | - |
5-methylpyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Saccharomyces cerevisiae | |
| 3.5.1.19 | 0.00085 | - |
5-methoxypyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Borreliella burgdorferi | |
| 3.5.1.19 | 0.001 | - |
4-methoxylnicotinaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 0.0014 | - |
nicotinaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Saccharomyces cerevisiae | |
| 3.5.1.19 | 0.0038 | - |
5-methoxypyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Saccharomyces cerevisiae | |
| 3.5.1.19 | 0.004 | - |
6-fluoropyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 0.004 | - |
5-bromopyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Saccharomyces cerevisiae | |
| 3.5.1.19 | 0.018 | - |
6-fluoropyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Borreliella burgdorferi | |
| 3.5.1.19 | 0.05 | - |
4-methoxylnicotinaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Streptococcus pneumoniae | |
| 3.5.1.19 | 0.068 | - |
4-methoxylnicotinaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Saccharomyces cerevisiae | |
| 3.5.1.19 | 0.085 | - |
3-Cyanopyridine | in 100 mM phosphate buffer, pH 7.3 at 25°C | Saccharomyces cerevisiae | |
| 3.5.1.19 | 0.153 | - |
4-methoxylnicotinaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Borreliella burgdorferi | |
| 3.5.1.19 | 0.37 | - |
2-chloropyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Borreliella burgdorferi | |
| 3.5.1.19 | 0.5 | - |
3-Cyanopyridine | in 100 mM phosphate buffer, pH 7.3 at 25°C | Streptococcus pneumoniae | |
| 3.5.1.19 | 1 | - |
3-Cyanopyridine | in 100 mM phosphate buffer, pH 7.3 at 25°C | Plasmodium falciparum | |
| 3.5.1.19 | 2 | - |
nicotinic acid | in 100 mM phosphate buffer, pH 7.3 at 25°C | Streptococcus pneumoniae | |
| 3.5.1.19 | 2 | - |
3-Cyanopyridine | in 100 mM phosphate buffer, pH 7.3 at 25°C | Borreliella burgdorferi | |
| 3.5.1.19 | 5 | - |
2-chloropyridine-3-carbaldehyde | in 100 mM phosphate buffer, pH 7.3 at 25°C | Saccharomyces cerevisiae | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | physiological function | consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability | Saccharomyces cerevisiae |
| 3.5.1.19 | physiological function | consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability | Streptococcus pneumoniae |
| 3.5.1.19 | physiological function | consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability | Caenorhabditis elegans |
| 3.5.1.19 | physiological function | consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability | Plasmodium falciparum |
| 3.5.1.19 | physiological function | consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability | Borreliella burgdorferi |
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Release 2023.1 (January 2023)
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