EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.2.6 | R39Q | titration with cis,cis-muconate shows negative cooperativity | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.2.6 | (2E)-2-fluoro-2,4-pentadienoate | - |
Pseudomonas putida | |
5.3.2.6 | (2E)-fluoromuconate | a dicarboxylate competitive inhibitor, shows substoichiometric binding to 3 +/- 1 sites per hexamer | Pseudomonas putida | |
5.3.2.6 | (2Z)-2-fluoro-2,4-pentadienoate | - |
Pseudomonas putida | |
5.3.2.6 | additional information | although 4-OT exhibits no structural asymmetry either by X-ray or by NMR, inactivation by two affinity labels shows half-site stoichiometry | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.2.6 | additional information | - |
additional information | 4-OT exhibits hyperbolic kinetics and shows anticooperativity during catalysis | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.2.6 | Pseudomonas putida | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.2.6 | 2-oxo-4-hexenedioate | the enzyme converts the unconjugated enone to the conjugated enone via a dienolic intermediate 2-hydroxymuconate, Pro1 serves as the general base, and both Arg11 and Arg39 function in substrate binding and catalysis in an otherwise hydrophobic active site. Anticooperativity during catalysis | Pseudomonas putida | 2-oxo-3-hexenedioate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.2.6 | homohexamer | - |
Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.2.6 | 4-OT | - |
Pseudomonas putida |
5.3.2.6 | 4-oxalocrotonate tautomerase | - |
Pseudomonas putida |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.2.6 | 0.045 | - |
(2E)-fluoromuconate | pH and temperature not specified in the publication | Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.3.2.6 | additional information | structure-function relationship and kinetic analysis, detailed overview | Pseudomonas putida |