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Literature summary extracted from

  • Zhang, Y.; Zhou, J.; Chang, M.; Bai, L.; Shan, J.; Yao, C.; Jiang, R.; Guo, L.; Zhang, R.; Wu, J.; Li, Y.
    Characterization of and functional evidence for Ste27 of Streptomyces sp. 139 as a novel spermine/spermidine acetyltransferase (2012), Biochem. J., 443, 727-734.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.57 expression in Escherichia coli Streptomyces sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.57 molecular modeling of structure. Both spermidine and spermine should bind at unique position with high specificity Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.57 0.056
-
 acetyl-CoA pH 8.0, 30°C Streptomyces sp.
2.3.1.57 0.065
-
 spermidine pH 8.0, 30°C Streptomyces sp.
2.3.1.57 0.215
-
 D-glucosamine 6-phosphate pH 8.0, 30°C Streptomyces sp.
2.3.1.57 0.338
-
 spermine pH 8.0, 30°C Streptomyces sp.
2.3.1.57 0.859
-
 poly-L-lysine pH 8.0, 30°C Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Streptomyces sp. D2CFQ9 putative
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 3 acetyl-CoA + spermidine spermidine is preferred over spermine Streptomyces sp. 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + D-glucosamine 6-phosphate
-
 Streptomyces sp. CoA + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.3.1.57 acetyl-CoA + poly-L-lysine
-
 Streptomyces sp. CoA + acetyl-poly-L-lysine
-
 ?
2.3.1.57 acetyl-CoA + spermine spermidine is preferred over spermine Streptomyces sp. CoA + N1-acetylspermine
-
 ?
2.3.1.57 additional information no substrates: glycine, glutamate, aspartate, leucine, alanine or arginine Streptomyces sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.57 Ste27
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.57 28
-
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.57 15
-
 about 50% of maximum activity Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.57 0.858
-
 acetyl-CoA pH 8.0, 30°C Streptomyces sp.
2.3.1.57 0.922
-
 spermidine pH 8.0, 30°C Streptomyces sp.
2.3.1.57 1.358
-
 D-glucosamine 6-phosphate pH 8.0, 30°C Streptomyces sp.
2.3.1.57 1.86
-
 spermine pH 8.0, 30°C Streptomyces sp.
2.3.1.57 3.953
-
 poly-L-lysine pH 8.0, 30°C Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.57 7.5
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.57 6
-
 about 50% of maximum activity Streptomyces sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.57 2.76
-
 spermidine pH 8.0, 30°C Streptomyces sp.
2.3.1.57 4.6
-
 poly-L-lysine pH 8.0, 30°C Streptomyces sp.
2.3.1.57 8.63
-
 spermine pH 8.0, 30°C Streptomyces sp.
2.3.1.57 13.17
-
 acetyl-CoA pH 8.0, 30°C Streptomyces sp.
2.3.1.57 24
-
 D-glucosamine 6-phosphate pH 8.0, 30°C Streptomyces sp.