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Literature summary extracted from

  • Zhang, Y.; Sun, K.; Sandoval, F.J.; Santiago, K.; Roje, S.
    One-carbon metabolism in plants: characterization of a plastid serine hydroxymethyltransferase (2010), Biochem. J., 430, 97-105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 phylogenetic analysis, expression in Escherichia coli strain Rosetta Hordeum vulgare
2.1.2.1 phylogenetic analysis, expression in Escherichia coli strain Rosetta, transient expression of EGFP-tagged full-length AtSHMT3 in Arabidopsis thaliana leaf protoplasts Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 additional information model of uncompetitive substrate inhibition using tetrahydropteroylglutamates with different numbers of glutamate residues, overview Arabidopsis thaliana
2.1.2.1 tetrahydropteroylglutamate
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.2177
-
 tetrahydrofolate pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 chloroplast
-
 Hordeum vulgare 9507
-
2.1.2.1 chloroplast
-
 Pisum sativum 9507
-
2.1.2.1 chloroplast AtSHMT3 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Hordeum vulgare
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Pisum sativum
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Arabidopsis thaliana
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Hordeum vulgare Bob
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Pisum sativum Progress 9
-
 tetrahydrofolate + L-serine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Arabidopsis thaliana Q94JQ3 ecotype Columbia
-
2.1.2.1 Hordeum vulgare
-
-
-
2.1.2.1 Hordeum vulgare Bob
-
-
-
2.1.2.1 Pisum sativum
-
-
-
2.1.2.1 Pisum sativum Progress 9
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 partial by chloroplast preparation Hordeum vulgare
2.1.2.1 partial by chloroplast preparation Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 flower
-
 Arabidopsis thaliana
-
2.1.2.1 leaf
-
 Hordeum vulgare
-
2.1.2.1 leaf
-
 Pisum sativum
-
2.1.2.1 rosette leaf
-
 Arabidopsis thaliana
-
2.1.2.1 seed germinated, high expression level of AtSHMT3 Arabidopsis thaliana
-
2.1.2.1 seedling
-
 Arabidopsis thaliana
-
2.1.2.1 silique
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Hordeum vulgare tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Pisum sativum tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Arabidopsis thaliana tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Arabidopsis thaliana tetrahydrofolate + L-serine i.e. tetrahydropteroylglutamate, tetrahydropteroylglutamates with more than one glutamate residue are poor substrates and competitive inhibitors, overview r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Hordeum vulgare Bob tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Pisum sativum Progress 9 tetrahydrofolate + L-serine
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 AtSHMT3
-
 Arabidopsis thaliana
2.1.2.1 serine hydroxymethyltransferase
-
 Hordeum vulgare
2.1.2.1 serine hydroxymethyltransferase
-
 Pisum sativum
2.1.2.1 serine hydroxymethyltransferase
-
 Arabidopsis thaliana
2.1.2.1 SHMT
-
 Hordeum vulgare
2.1.2.1 SHMT
-
 Pisum sativum
2.1.2.1 SHMT
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 15.8
-
 tetrahydrofolate pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 8.5
-
 assay at Hordeum vulgare
2.1.2.1 8.5
-
 assay at Pisum sativum
2.1.2.1 8.5
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate dependent on Hordeum vulgare
2.1.2.1 pyridoxal 5'-phosphate dependent on Pisum sativum
2.1.2.1 pyridoxal 5'-phosphate dependent on Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.1 0.085
-
 tetrahydropteroylglutamate pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.1.2.1 physiological function in plastids, SHMTs are thought to catalytically direct the hydroxymethyl moiety of serine into the metabolic network of H4PteGlun-bound one-carbon units Hordeum vulgare
2.1.2.1 physiological function in plastids, SHMTs are thought to catalytically direct the hydroxymethyl moiety of serine into the metabolic network of H4PteGlun-bound one-carbon units Pisum sativum
2.1.2.1 physiological function in plastids, SHMTs are thought to catalytically direct the hydroxymethyl moiety of serine into the metabolic network of H4PteGlun-bound one-carbon units Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.2.1 70
-
 tetrahydrofolate pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 Arabidopsis thaliana