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Literature summary extracted from

  • Jiang, Y.; Chen, X.M.; Liu, Y.J.; Li, Y.T.; Zhang, H.H.; Dyson, P.; Sheng, H.M.; An, L.Z.
    The catalytic efficiency of trehalose-6-phosphate synthase is effected by the N-loop at low temperatures (2010), Arch. Microbiol., 192, 937-943.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15 gene otsA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B21(DE3) Arthrobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.15 additional information construction of N-loop exchange mutations of the otsA gene, the mutants show altered catalytic efficiencies at temperatures from 16 to 37°C compared to the wild-type enzyme, overview Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.15 additional information
-
 additional information the high catalytic efficiency of OtsA results from the high affinity of the enzyme for uridine 5'-diphosphoglucose at low temperatures Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate Arthrobacter sp.
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate Arthrobacter sp. Cjts
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Arthrobacter sp. Q1KLW0 gene otsA
-
2.4.1.15 Arthrobacter sp. Cjts Q1KLW0 gene otsA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.15 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain B21(DE3) by nickel affinity chromatography Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Arthrobacter sp. UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Arthrobacter sp. Cjts UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.15 OtsA
-
 Arthrobacter sp.
2.4.1.15 trehalose-6-phosphate synthase
-
 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.15 37
-
 assay at Arthrobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.15 16 37 tested activity range Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.15 7.5
-
 assay at Arthrobacter sp.

General Information

EC Number General Information Comment Organism
2.4.1.15 physiological function the multifunctional protein, OtsA, is indispensable for bacterial survival in various environments. The catalytic efficiency of OtsA is affected by its N-loop at low temperatures. The flexibility of the OtsA N-loop is related to the growth temperature of the respective bacterial species Arthrobacter sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.15 additional information
-
 additional information the high catalytic efficiency of OtsA results from the high affinity of the enzyme for uridine 5'-diphosphoglucose at low temperatures Arthrobacter sp.