EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.37 | expressed in Escherichia coli | Mus musculus |
2.3.1.47 | His-tagged protein and murine 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer) expressed in Escherichia coli HU227, R872 and DH5alpha | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.37 | additional information | a single chain dimeric ALAS variant is created, in which one of the two active sites harbors a K313A mutation eliminating measurable enzyme activity in order to investigate the unusual enhanced enzymatic activity resulting from linking ALAS dimmers. The two active sites in ALAS/ALAS differentially contribute to the enhanced activity of the enzyme, even though the amount of ALA produced during the first turnover is identical in both active sites. The kcat values of the K313A variants differ significantly depending on which of the two active sites harbors the mutation | Mus musculus |
2.3.1.47 | K236A | active site lysine involved in the Schiff base linkage with the PLP cofactor | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.37 | 0.00032 | - |
glycine | pH 7.5, 30°C, ALAS(K313A mutant)/ALAS | Mus musculus | |
2.3.1.37 | 0.00045 | - |
glycine | pH 7.5, 30°C, ALAS/ALAS | Mus musculus | |
2.3.1.37 | 0.0018 | - |
glycine | pH 7.5, 30°C, ALAS/ALAS (K313A mutant) | Mus musculus | |
2.3.1.37 | 0.002 | - |
glycine | pH 7.5, 30°C, ALAS | Mus musculus | |
2.3.1.37 | 2 | 3 | glycine | pH 7.5, 30°C, ALAS | Mus musculus | |
2.3.1.37 | 11.8 | - |
glycine | pH 7.5, 30°C, ALAS/ALAS (K313A mutant) | Mus musculus | |
2.3.1.37 | 14.8 | - |
glycine | pH 7.5, 30°C, ALAS(K313A mutant)/ALAS | Mus musculus | |
2.3.1.37 | 16.7 | - |
glycine | pH 7.5, 30°C, ALAS/ALAS | Mus musculus | |
2.3.1.47 | 0.01 | - |
pimeloyl-CoA | 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C | Escherichia coli | |
2.3.1.47 | 0.025 | - |
pimeloyl-CoA | wild type protein, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2.3.1.47 | 0.25 | - |
L-alanine | 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C | Escherichia coli | |
2.3.1.47 | 0.5 | - |
L-alanine | wild type protein, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.37 | Mus musculus | - |
- |
- |
2.3.1.47 | Escherichia coli | P12998 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.47 | from Escherichia coli DH5alpha, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, ion exchange chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.37 | succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? | |
2.3.1.47 | L-alanine + pimeloyl-CoA | - |
Escherichia coli | 8-amino-7-oxononanoate + CoA + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.47 | homodimer | 2 x 40000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.37 | 5-aminolevulinate synthase | - |
Mus musculus |
2.3.1.37 | ALAS | - |
Mus musculus |
2.3.1.47 | 8-amino-7-oxononanoate synthase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.37 | 30 | - |
assay at | Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.37 | 0.07 | - |
glycine | pH 7.5, 30°C, ALAS/ALAS (K313A mutant) | Mus musculus | |
2.3.1.37 | 0.166 | - |
glycine | pH 7.5, 30°C, ALAS | Mus musculus | |
2.3.1.37 | 0.36 | - |
glycine | pH 7.5, 30°C, ALAS(K313A mutant)/ALAS | Mus musculus | |
2.3.1.37 | 0.92 | - |
glycine | pH 7.5, 30°C, ALAS/ALAS | Mus musculus | |
2.3.1.47 | 0.028 | - |
L-alanine | 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C | Escherichia coli | |
2.3.1.47 | 0.06 | - |
L-alanine | wild type protein, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.37 | 7.5 | - |
assay at | Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.37 | pyridoxal 5'-phosphate | - |
Mus musculus | |
2.3.1.47 | pyridoxal 5'-phosphate | - |
Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.47 | 0.11 | - |
L-alanine | 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C | Escherichia coli | |
2.3.1.47 | 0.12 | - |
L-alanine | wild type protein, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2.3.1.47 | 2.33 | - |
pimeloyl-CoA | wild type protein, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2.3.1.47 | 2.67 | - |
pimeloyl-CoA | 5-aminolevulinate synthase-fusion protein (single chain chimeric dimer), pH 7.5, 30°C | Escherichia coli |