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Literature summary extracted from

  • Huang, S.Y.; Zhang, Y.H.; Zhong, J.J.
    A thermostable recombinant transaldolase with high activity over a broad pH range (2012), Appl. Microbiol. Biotechnol., 93, 2403-2410.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.2 expression in Escherichia coli Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.2 0.27
-
 D-erythrose 4-phosphate pH 9.0, 80°C Thermotoga maritima
2.2.1.2 0.36
-
 D-fructose 6-phosphate pH 9.0, 80°C Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.2 24000
-
 x * 24000, SDS-PAGE, x * 24213, calculated Thermotoga maritima
2.2.1.2 24213
-
 x * 24000, SDS-PAGE, x * 24213, calculated Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.2 Thermotoga maritima Q9WYD1
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.2 8.9
-
 30°C, pH 9.0 Thermotoga maritima
2.2.1.2 42
-
 80°C, pH 9.0 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate
-
 Thermotoga maritima sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.2 ? x * 24000, SDS-PAGE, x * 24213, calculated Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.2 80
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.2.1.2 40
-
 28% of maximum activity Thermotoga maritima
2.2.1.2 60
-
 73% of maximum activity Thermotoga maritima
2.2.1.2 90
-
 96% of maximum activity Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.2 60
-
 half life 198 h Thermotoga maritima
2.2.1.2 80
-
 half-life 13 h Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.2 22.3
-
 D-fructose 6-phosphate pH 9.0, 80°C Thermotoga maritima
2.2.1.2 22.9
-
 D-erythrose 4-phosphate pH 9.0, 80°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.2 9
-
 in glycine-NaOH buffer Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.2 6 9
-
 Thermotoga maritima