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Literature summary extracted from

  • Yang, T.H.; Jung, Y.K.; Kang, H.O.; Kim, T.W.; Park, S.J.; Lee, S.Y.
    Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli (2011), Appl. Microbiol. Biotechnol., 90, 603-614.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 gene phaC1Ps6-19 phylogenetic analysis, expression of phaC1Ps6-19 mutants in Escherichia coli strain XL-1 Blue Pseudomonas aeruginosa
2.3.1.304 gene phaC1Ps6-19 phylogenetic analysis, expression of phaC1Ps6-19 mutants in Escherichia coli strain XL-1 Blue Pseudomonas putida
2.3.1.304 gene phaC1Ps6-19 phylogenetic analysis, expression of phaC1Ps6-19 mutants in Escherichia coli strain XL-1 Blue Pseudomonas resinovorans
2.3.1.304 gene phaC1Ps6-19 phylogenetic analysis, expression of phaC1Ps6-19 mutants in Escherichia coli strain XL-1 Blue Pseudomonas sp.
2.3.1.304 gene phaC1Ps6-19, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of mutant E130D/S325T/S477G/Q481K in Escherichia coli strain XL-1 Blue Pseudomonas chlororaphis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 E130D/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas aeruginosa
2.3.1.304 E130D/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas putida
2.3.1.304 E130D/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas resinovorans
2.3.1.304 E130D/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas sp.
2.3.1.304 E130D/S325T/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas aeruginosa
2.3.1.304 E130D/S325T/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas putida
2.3.1.304 E130D/S325T/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas resinovorans
2.3.1.304 E130D/S325T/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas sp.
2.3.1.304 E130D/S325T/S477G/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas aeruginosa
2.3.1.304 E130D/S325T/S477G/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas putida
2.3.1.304 E130D/S325T/S477G/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas resinovorans
2.3.1.304 E130D/S325T/S477G/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas sp.
2.3.1.304 E130D/S325T/S477G/Q481K site-directed mutagenesis, the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481 Pseudomonas aeruginosa
2.3.1.304 E130D/S325T/S477G/Q481K site-directed mutagenesis, the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481 Pseudomonas putida
2.3.1.304 E130D/S325T/S477G/Q481K site-directed mutagenesis, the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481 Pseudomonas resinovorans
2.3.1.304 E130D/S325T/S477G/Q481K site-directed mutagenesis, the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481 Pseudomonas sp.
2.3.1.304 E130D/S325T/S477G/Q481K the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481 Pseudomonas chlororaphis
2.3.1.304 E130D/S477F/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas aeruginosa
2.3.1.304 E130D/S477F/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas putida
2.3.1.304 E130D/S477F/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas resinovorans
2.3.1.304 E130D/S477F/Q481K site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA) Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas aeruginosa
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas putida
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas chlororaphis
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas resinovorans
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas sp.
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas chlororaphis KCTC 12349
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas putida KT 2240
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas resinovorans KCTC 12498
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Pseudomonas aeruginosa
-
 KCTC 1637, gene phaC1Ps6-19
-
2.3.1.304 Pseudomonas chlororaphis C0LD26 gene phaC1Ps6-19
-
2.3.1.304 Pseudomonas chlororaphis KCTC 12349 C0LD26 gene phaC1Ps6-19
-
2.3.1.304 Pseudomonas putida
-
 ATCC 47054, gene phaC1Ps6-19
-
2.3.1.304 Pseudomonas putida KT 2240
-
 ATCC 47054, gene phaC1Ps6-19
-
2.3.1.304 Pseudomonas resinovorans Q9X5X7 gene phaC1Ps6-19
-
2.3.1.304 Pseudomonas resinovorans KCTC 12498 Q9X5X7 gene phaC1Ps6-19
-
2.3.1.304 Pseudomonas sp. Q9Z3Y1 gene phaC1Ps6-19
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Pseudomonas aeruginosa [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Pseudomonas putida [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Pseudomonas chlororaphis [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Pseudomonas resinovorans [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Pseudomonas sp. [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Pseudomonas chlororaphis KCTC 12349 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Pseudomonas putida KT 2240 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
2.3.1.304 3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Pseudomonas resinovorans KCTC 12498 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 type II PHA synthase
-
 Pseudomonas aeruginosa
2.3.1.304 type II PHA synthase
-
 Pseudomonas putida
2.3.1.304 type II PHA synthase
-
 Pseudomonas chlororaphis
2.3.1.304 type II PHA synthase
-
 Pseudomonas resinovorans
2.3.1.304 type II PHA synthase
-
 Pseudomonas sp.
2.3.1.304 type II PhaC1
-
 Pseudomonas aeruginosa
2.3.1.304 type II PhaC1
-
 Pseudomonas putida
2.3.1.304 type II PhaC1
-
 Pseudomonas chlororaphis
2.3.1.304 type II PhaC1
-
 Pseudomonas resinovorans
2.3.1.304 type II PhaC1
-
 Pseudomonas sp.
2.3.1.304 type II Pseudomonas PHA synthase 1
-
 Pseudomonas aeruginosa
2.3.1.304 type II Pseudomonas PHA synthase 1
-
 Pseudomonas putida
2.3.1.304 type II Pseudomonas PHA synthase 1
-
 Pseudomonas chlororaphis
2.3.1.304 type II Pseudomonas PHA synthase 1
-
 Pseudomonas resinovorans
2.3.1.304 type II Pseudomonas PHA synthase 1
-
 Pseudomonas sp.