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Literature summary extracted from

  • Qiu, J.; Ma, Y.; Wen, Y.; Chen, L.; Wu, L.; Liu, W.
    Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine (2012), Appl. Environ. Microbiol., 78, 2154-2160.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.83 expression in Escherichia coli Pseudomonas sp.
1.4.3.24 expression in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.24 Ag+ strong inhibition Pseudomonas sp.
1.4.3.24 Co2+ strong inhibition Pseudomonas sp.
1.4.3.24 Cu2+ strong inhibition Pseudomonas sp.
1.4.3.24 additional information not inhibitory: Ca2+, Mg2+, Mn2+, Ni2+, Zn2+ Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.24 0.247
-
4-(methylamino)-1-(pyridin-3-yl)butan-1-one pH 8.0, temperature not specified in the publication Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.24 54101
-
x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein Pseudomonas sp.
1.4.3.24 55000
-
x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.83 Pseudomonas sp. H8ZPX2
-
-
1.2.1.83 Pseudomonas sp. HZN6 H8ZPX2
-
-
1.4.3.24 Pseudomonas sp. H8ZPX1
-
-
1.4.3.24 Pseudomonas sp. HZN6 H8ZPX1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.83 4-oxo-4-(pyridin-3-yl)butanal + NADP+ + H2O
-
Pseudomonas sp. 4-oxo-4-(pyridin-3-yl)butanoate + NADPH + H+
-
?
1.2.1.83 4-oxo-4-(pyridin-3-yl)butanal + NADP+ + H2O
-
Pseudomonas sp. HZN6 4-oxo-4-(pyridin-3-yl)butanoate + NADPH + H+
-
?
1.4.3.24 4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2
-
Pseudomonas sp. 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2
-
?
1.4.3.24 4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2
-
Pseudomonas sp. HZN6 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2
-
?
1.4.3.24 additional information no substrates: nicotine, gamma-N-methylaminobutanoate, gamma-aminobutanoate, spermidine, spermine, sarcosine, methylamine, dimethylamine, dimethylglycine, and 3-(methylamino)propylamine Pseudomonas sp. ?
-
?
1.4.3.24 additional information no substrates: nicotine, gamma-N-methylaminobutanoate, gamma-aminobutanoate, spermidine, spermine, sarcosine, methylamine, dimethylamine, dimethylglycine, and 3-(methylamino)propylamine Pseudomonas sp. HZN6 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.24 ? x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.83 ALD
-
Pseudomonas sp.
1.2.1.83 SapD
-
Pseudomonas sp.
1.4.3.24 PAO
-
Pseudomonas sp.
1.4.3.24 pseudooxynicotine amine oxidase
-
Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.24 35
-
-
Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.24 151
-
4-(methylamino)-1-(pyridin-3-yl)butan-1-one pH 8.0, temperature not specified in the publication Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.24 8
-
-
Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.83 additional information no cofactor: NAD+ Pseudomonas sp.
1.2.1.83 NADP+ specific for NADP+ Pseudomonas sp.
1.4.3.24 FAD noncovalently bound, 0.5 mol per mol of subunit Pseudomonas sp.
1.4.3.24 additional information no FMN detected Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.2.1.83 physiological function an enzyme deletion strain is still able to grow on pseudoxynicotine, the cell growth and the rate of pseudoxynicotine removal are initially slightly reduced. After the deletion strain is cultured with pseudoxynicotine for several generations, the pseudoxynicotine degradation characteristics reach the wild-type level Pseudomonas sp.
1.4.3.24 physiological function gene disruption mutant strain is unable to use nicotine as the sole carbon source and accumulates pseudooxynicotine Pseudomonas sp.