EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.83 | expression in Escherichia coli | Pseudomonas sp. |
1.4.3.24 | expression in Escherichia coli | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.24 | Ag+ | strong inhibition | Pseudomonas sp. | |
1.4.3.24 | Co2+ | strong inhibition | Pseudomonas sp. | |
1.4.3.24 | Cu2+ | strong inhibition | Pseudomonas sp. | |
1.4.3.24 | additional information | not inhibitory: Ca2+, Mg2+, Mn2+, Ni2+, Zn2+ | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.24 | 0.247 | - |
4-(methylamino)-1-(pyridin-3-yl)butan-1-one | pH 8.0, temperature not specified in the publication | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.3.24 | 54101 | - |
x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein | Pseudomonas sp. |
1.4.3.24 | 55000 | - |
x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.83 | Pseudomonas sp. | H8ZPX2 | - |
- |
1.2.1.83 | Pseudomonas sp. HZN6 | H8ZPX2 | - |
- |
1.4.3.24 | Pseudomonas sp. | H8ZPX1 | - |
- |
1.4.3.24 | Pseudomonas sp. HZN6 | H8ZPX1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.83 | 4-oxo-4-(pyridin-3-yl)butanal + NADP+ + H2O | - |
Pseudomonas sp. | 4-oxo-4-(pyridin-3-yl)butanoate + NADPH + H+ | - |
? | |
1.2.1.83 | 4-oxo-4-(pyridin-3-yl)butanal + NADP+ + H2O | - |
Pseudomonas sp. HZN6 | 4-oxo-4-(pyridin-3-yl)butanoate + NADPH + H+ | - |
? | |
1.4.3.24 | 4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2 | - |
Pseudomonas sp. | 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2 | - |
? | |
1.4.3.24 | 4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2 | - |
Pseudomonas sp. HZN6 | 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2 | - |
? | |
1.4.3.24 | additional information | no substrates: nicotine, gamma-N-methylaminobutanoate, gamma-aminobutanoate, spermidine, spermine, sarcosine, methylamine, dimethylamine, dimethylglycine, and 3-(methylamino)propylamine | Pseudomonas sp. | ? | - |
? | |
1.4.3.24 | additional information | no substrates: nicotine, gamma-N-methylaminobutanoate, gamma-aminobutanoate, spermidine, spermine, sarcosine, methylamine, dimethylamine, dimethylglycine, and 3-(methylamino)propylamine | Pseudomonas sp. HZN6 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.3.24 | ? | x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein | Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.83 | ALD | - |
Pseudomonas sp. |
1.2.1.83 | SapD | - |
Pseudomonas sp. |
1.4.3.24 | PAO | - |
Pseudomonas sp. |
1.4.3.24 | pseudooxynicotine amine oxidase | - |
Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.24 | 35 | - |
- |
Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.24 | 151 | - |
4-(methylamino)-1-(pyridin-3-yl)butan-1-one | pH 8.0, temperature not specified in the publication | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.24 | 8 | - |
- |
Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.83 | additional information | no cofactor: NAD+ | Pseudomonas sp. | |
1.2.1.83 | NADP+ | specific for NADP+ | Pseudomonas sp. | |
1.4.3.24 | FAD | noncovalently bound, 0.5 mol per mol of subunit | Pseudomonas sp. | |
1.4.3.24 | additional information | no FMN detected | Pseudomonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.1.83 | physiological function | an enzyme deletion strain is still able to grow on pseudoxynicotine, the cell growth and the rate of pseudoxynicotine removal are initially slightly reduced. After the deletion strain is cultured with pseudoxynicotine for several generations, the pseudoxynicotine degradation characteristics reach the wild-type level | Pseudomonas sp. |
1.4.3.24 | physiological function | gene disruption mutant strain is unable to use nicotine as the sole carbon source and accumulates pseudooxynicotine | Pseudomonas sp. |