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Literature summary extracted from

  • Hansen, L.H.; Planellas, M.H.; Long, K.S.; Vester, B.
    The order Bacillales hosts functional homologs of the worrisome cfr antibiotic resistance gene (2012), Antimicrob. Agents Chemother., 56, 3563-3567.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.224 gene clbA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10 Bacillus amyloliquefaciens
2.1.1.224 gene clbB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10 Brevibacillus brevis
2.1.1.224 gene clbC, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10 Alkalihalobacillus clausii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Bacillus amyloliquefaciens
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Brevibacillus brevis
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Alkalihalobacillus clausii
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Bacillus amyloliquefaciens FZB42
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Brevibacillus brevis NBRC 100599
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.224 Alkalihalobacillus clausii Q5WJ42 or strain domuvar, gene clbC, a cfr-like gene
-
2.1.1.224 Bacillus amyloliquefaciens
-
 gene clbA, a cfr-like gene
-
2.1.1.224 Bacillus amyloliquefaciens FZB42
-
 gene clbA, a cfr-like gene
-
2.1.1.224 Brevibacillus brevis C0ZJA6 gene clbB, a cfr-like gene
-
2.1.1.224 Brevibacillus brevis NBRC 100599 C0ZJA6 gene clbB, a cfr-like gene
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Bacillus amyloliquefaciens S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Brevibacillus brevis S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Alkalihalobacillus clausii S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Bacillus amyloliquefaciens S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Brevibacillus brevis S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Alkalihalobacillus clausii S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Bacillus amyloliquefaciens FZB42 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Bacillus amyloliquefaciens FZB42 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Brevibacillus brevis NBRC 100599 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Brevibacillus brevis NBRC 100599 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.224 ClbA
-
 Bacillus amyloliquefaciens
2.1.1.224 ClbB
-
 Brevibacillus brevis
2.1.1.224 ClbC
-
 Alkalihalobacillus clausii

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.224 S-adenosyl-L-methionine
-
 Bacillus amyloliquefaciens
2.1.1.224 S-adenosyl-L-methionine
-
 Brevibacillus brevis
2.1.1.224 S-adenosyl-L-methionine
-
 Alkalihalobacillus clausii

General Information

EC Number General Information Comment Organism
2.1.1.224 evolution ClbA acts via the same mechanism as the Cfr methyltransferase Bacillus amyloliquefaciens
2.1.1.224 evolution ClbB acts via the same mechanism as the Cfr methyltransferase Brevibacillus brevis
2.1.1.224 evolution ClbC acts via the same mechanism as the Cfr methyltransferase Alkalihalobacillus clausii
2.1.1.224 physiological function ClbA confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview Bacillus amyloliquefaciens
2.1.1.224 physiological function ClbB confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview Brevibacillus brevis
2.1.1.224 physiological function ClbC confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview Alkalihalobacillus clausii