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Literature summary extracted from

  • Mateo, C.; Chmura, A.; Rustler, S.; van Rantwijk, F.; Stolz, A.; Sheldon, R.A.
    Synthesis of enantiomerically pure (S)-mandelic acid using anoxynitrilasenitrilase bienzymatic cascade: a nitrilase surprisingly shows nitrile hydratase activity (2006), Tetrahedron Asymmetry, 17, 320-323.
No PubMed abstract available

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.5 Pseudomonas fluorescens Q5EG61
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3.5.5.5 Pseudomonas fluorescens EBC191 Q5EG61
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.5 (S)-mandelonitrile + 2 H2O
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 Pseudomonas fluorescens (S)-mandelic acid + NH3 the product is 94% enantiomeric pure (S)-isomer, the formation of ca 50% (S)-mandeloamide is observed ?
3.5.5.5 (S)-mandelonitrile + 2 H2O
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 Pseudomonas fluorescens EBC191 (S)-mandelic acid + NH3 the product is 94% enantiomeric pure (S)-isomer, the formation of ca 50% (S)-mandeloamide is observed ?

General Information

EC Number General Information Comment Organism
3.5.5.5 metabolism involved in the biosynthesis of adenosylcobalamin Pseudomonas fluorescens