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Literature summary extracted from

  • Jirschitzka, J.; Schmidt, G.W.; Reichelt, M.; Schneider, B.; Gershenzon, J.; D'Auria, J.C.
    Plant tropane alkaloid biosynthesis evolved independently in the Solanaceae and Erythroxylaceae (2012), Proc. Natl. Acad. Sci. USA, 109, 10304-10309.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.334 expressed in Escherichia coli Erythroxylum coca

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.334 Co2+ 5 mM, inhibition by less than 30% Erythroxylum coca
1.1.1.334 Cu2+ 5 mM, 8% residual activity Erythroxylum coca
1.1.1.334 Fe2+ 5 mM, inhibition by less than 30% Erythroxylum coca
1.1.1.334 methyl ecgonine
-
 Erythroxylum coca
1.1.1.334 methyl ecgonone
-
 Erythroxylum coca
1.1.1.334 NADP+
-
 Erythroxylum coca
1.1.1.334 NADPH
-
 Erythroxylum coca
1.1.1.334 Zn2+ 5 mM, 58% residual activity Erythroxylum coca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.334 0.0009
-
 NADPH pH 6.8, temperature not specified in the publication Erythroxylum coca
1.1.1.334 0.0009
-
 NADP+ pH 6.8, temperature not specified in the publication Erythroxylum coca
1.1.1.334 0.069
-
 methyl ecgonone pH 6.8, temperature not specified in the publication Erythroxylum coca
1.1.1.334 0.151
-
 methyl ecgonine pH 6.8, temperature not specified in the publication Erythroxylum coca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.334 additional information not activated by monovalent or divalent cations Erythroxylum coca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.334 36910
-
 calculated from the amino acid sequence Erythroxylum coca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.334 methyl ecgonone + NADPH + H+ Erythroxylum coca cocaine biosynthesis methyl ecgonine + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.334 Erythroxylum coca E7C196
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.334
-
 Erythroxylum coca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.334 flower less than 20% of activity compared to young leaves Erythroxylum coca
-
1.1.1.334 leaf highest activity in young leaves Erythroxylum coca
-
1.1.1.334 mesophyll of young leaves Erythroxylum coca
-
1.1.1.334 additional information no activity in mature leaves or in roots Erythroxylum coca
-
1.1.1.334 seed
-
 Erythroxylum coca
-
1.1.1.334 stem less than 20% of activity compared to young leaves Erythroxylum coca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.334 0.0002669
-
 pH 6.8 temperature not specified in the publication Erythroxylum coca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.334 6-hydroxytropinone + NADPH + H+ reduction at 45% of the activity with methylecgonone Erythroxylum coca ? + NADP+
-
 r
1.1.1.334 methyl ecgonone + NADH + H+ NADH can substitute for NADPH with 14% of the activity compared to NADPH Erythroxylum coca methyl ecgonine + NAD+
-
 r
1.1.1.334 methyl ecgonone + NADPH + H+
-
 Erythroxylum coca methyl ecgonine + NADP+
-
 r
1.1.1.334 methyl ecgonone + NADPH + H+ cocaine biosynthesis Erythroxylum coca methyl ecgonine + NADP+
-
 r
1.1.1.334 additional information no reduction of 8-thiabicyclo[3.2.1]octan-3-one, cyclohexanone, cyclooctanone, N-methyl-4-piperidone Erythroxylum coca ?
-
 ?
1.1.1.334 nortropinone + NADPH + H+ reduction at 6% of the activity with methylecgonone Erythroxylum coca ? + NADP+
-
 r
1.1.1.334 tropinone + NADPH + H+ reduction at 36% of the activity with methylecgonone Erythroxylum coca pseudotropine + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.334 MecgoR
-
 Erythroxylum coca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.334 25
-
 30 min, more than 30% loss of activity Erythroxylum coca
1.1.1.334 37
-
 30 min, more than 70% loss of activity Erythroxylum coca
1.1.1.334 50
-
 30 min, complete inactivation abvoe 50°C Erythroxylum coca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.334 6.8
-
 reduction of methyl ecgonone Erythroxylum coca
1.1.1.334 9.8
-
 oxidation of methyl ecgonine Erythroxylum coca

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.334 NADH 14% of the activity with NADPH Erythroxylum coca
1.1.1.334 NADPH
-
 Erythroxylum coca

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.334 0.001136
-
 methyl ecgonone substrate inhibition, pH 6.8, temperature not specified in the publication Erythroxylum coca
1.1.1.334 0.00248
-
 NADPH substrate inhibition, pH 6.8, temperature not specified in the publication Erythroxylum coca
1.1.1.334 0.002673
-
 methyl ecgonine substrate inhibition, pH 6.8, temperature not specified in the publication Erythroxylum coca
1.1.1.334 0.506
-
 NADP+ substrate inhibition, pH 6.8, temperature not specified in the publication Erythroxylum coca