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Literature summary extracted from

  • Necchi, F.; Nardi-Dei, V.; Biagini, M.; Assfalg, M.; Nuccitelli, A.; Cozzi, R.; Norais, N.; Telford, J.L.; Rinaudo, C.D.; Grandi, G.; Maione, D.
    Sortase A substrate specificity in GBS pilus 2a cell wall anchoring (2011), PLoS ONE, 6, e25300.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.70 23900
-
 x * 23900, truncated enzyme, calculated from amino acid sequence Staphylococcus aureus
3.4.22.70 25000
-
 x * 25000, truncated enzyme, SDS-PAGE Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Staphylococcus aureus
-
-
-
3.4.22.70 Staphylococcus aureus 515
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70 HiTrap Chelating HP column chromatography and Superdex 75 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 Dabcyl-SFLPKTGM-EDANS + H2O the substrate mimicks the LPXTG motif of pilus 2a minor ancillary protein Staphylococcus aureus ?
-
 ?
3.4.22.70 Dabcyl-SFLPKTGM-EDANS + H2O the substrate mimicks the LPXTG motif of pilus 2a minor ancillary protein Staphylococcus aureus 515 ?
-
 ?
3.4.22.70 additional information no activity with Dabcyl-KGIIPKTGGK-Edans, Dabcyl-KKVTIPQTGGIGT-Edans and Dabcyl-SFIPKTGM-Edans Staphylococcus aureus ?
-
 ?
3.4.22.70 additional information truncated SrtADELTAN40 also catalyzes in vitro transpeptidation reaction Staphylococcus aureus ?
-
 ?
3.4.22.70 additional information no activity with Dabcyl-KGIIPKTGGK-Edans, Dabcyl-KKVTIPQTGGIGT-Edans and Dabcyl-SFIPKTGM-Edans Staphylococcus aureus 515 ?
-
 ?
3.4.22.70 additional information truncated SrtADELTAN40 also catalyzes in vitro transpeptidation reaction Staphylococcus aureus 515 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.70 ? x * 23900, truncated enzyme, calculated from amino acid sequence Staphylococcus aureus
3.4.22.70 ? x * 25000, truncated enzyme, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.70 SrtA
-
 Staphylococcus aureus