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Literature summary extracted from

  • Chen, F.; Ro, D.K.; Petri, J.; Gershenzon, J.; Bohlmann, J.; Pichersky, E.; Tholl, D.
    Characterization of a root-specific Arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineole (2004), Plant Physiol., 135, 1956-1966.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.108 expression in Escherichia coli Arabidopsis thaliana
4.2.3.109 expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.108 0.0002
-
 geranyl diphosphate temperature not specified in the publication, pH not specified in the publication Arabidopsis thaliana
4.2.3.109 0.0002
-
 geranyl diphosphate temperature not specified in the publication, pH not specified in the publication Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.108 Mg2+ or Mn2+, absolutely required. Fully active at 40 mM Arabidopsis thaliana
4.2.3.108 Mn2+ or Mg2+, absolutely required. Fully active at 1 mM Arabidopsis thaliana
4.2.3.109 Mg2+ or Mn2+, absolutely required. Fully active at 40 mM Arabidopsis thaliana
4.2.3.109 Mn2+ or Mg2+, absolutely required. Fully active at 1 mM Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.108 60000
-
 x * 60000, SDS-PAGE Arabidopsis thaliana
4.2.3.109 60000
-
 x * 60000, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.108 Arabidopsis thaliana P0DI76
-
-
4.2.3.108 Arabidopsis thaliana P0DI77 isoform 2
-
4.2.3.109 Arabidopsis thaliana P0DI76
-
-
4.2.3.109 Arabidopsis thaliana P0DI77 isoform 2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.108 epidermis promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone Arabidopsis thaliana
-
4.2.3.108 root promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone Arabidopsis thaliana
-
4.2.3.108 stele promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone Arabidopsis thaliana
-
4.2.3.109 epidermis promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone Arabidopsis thaliana
-
4.2.3.109 root promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone Arabidopsis thaliana
-
4.2.3.109 stele promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.108 geranyl diphosphate + H2O
-
 Arabidopsis thaliana 1,8-cineole + diphosphate 52% 1,8-cineole plus 0.6% (+)-alpha-thujene, 1.9% (2)-(1S)-alpha-pinene, 14.5% (-)-sabinene, 7.8% (2)-(1S)-beta-pinene, 13.3% myrcene, 4.0% (2)-(4S)-limonene, (E)-2.7% beta-ocimene, 0.8% terpinolene, and 2.4% alpha-terpineol ?
4.2.3.108 additional information no substrate: farnesyl diphosphate Arabidopsis thaliana ?
-
 ?
4.2.3.109 geranyl diphosphate + H2O
-
 Arabidopsis thaliana (-)-sabinene + diphosphate 52% 1,8-cineole plus 0.6% (+)-alpha-thujene, 1.9% (2)-(1S)-alpha-pinene, 14.5% (-)-sabinene, 7.8% (2)-(1S)-beta-pinene, 13.3% myrcene, 4.0% (2)-(4S)-limonene, (E)-2.7% beta-ocimene, 0.8% terpinolene, and 2.4% alpha-terpineol ?
4.2.3.109 additional information no substrate: farnesyl diphosphate Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.108 ? x * 60000, SDS-PAGE Arabidopsis thaliana
4.2.3.109 ? x * 60000, SDS-PAGE Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.108 0.001
-
 geranyl diphosphate temperature not specified in the publication, pH not specified in the publication Arabidopsis thaliana
4.2.3.109 0.001
-
 geranyl diphosphate temperature not specified in the publication, pH not specified in the publication Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.108 8
-
-
 Arabidopsis thaliana
4.2.3.109 8
-
-
 Arabidopsis thaliana