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Literature summary extracted from

  • Demissie, Z.A.; Cella, M.A.; Sarker, L.S.; Thompson, T.J.; Rheault, M.R.; Mahmoud, S.S.
    Cloning, functional characterization and genomic organization of 1,8-cineole synthases from Lavandula (2012), Plant Mol. Biol., 79, 393-411.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.108 expression in Escherichia coli Lavandula x intermedia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.108 0.0057
-
 geranyl diphosphate pH 7.0, 30°C Lavandula x intermedia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.108 63000
-
 x * 63000, SDS-PAGE, x * 68500, calculated Lavandula x intermedia
4.2.3.108 68500
-
 x * 63000, SDS-PAGE, x * 68500, calculated Lavandula x intermedia

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.108 Lavandula x intermedia I3WEV8 parents Lavandula angustifolia and Lavandula latifolia
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.3.108 proteolytic modification the N-terminal 52 amino acids are predicted to code for a transit peptide Lavandula x intermedia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.108 geranyl diphosphate + H2O
-
 Lavandula x intermedia 1,8-cineole + diphosphate 80% 1,8-cineole plus 7.9% sabinene, 6.6% alpha-phellandrene, 2.3% limonene, and 1.7% alpha-terpineol ?
4.2.3.108 neryl diphosphate + H2O
-
 Lavandula x intermedia 1,8-cineole + diphosphate 61% 1,8-cineole plus 18.5% sabinene, 8% alpha-phellandrene, 3.3% limonene, and 5.5% alpha-terpineol ?

Subunits

EC Number Subunits Comment Organism
4.2.3.108 ? x * 63000, SDS-PAGE, x * 68500, calculated Lavandula x intermedia

Synonyms

EC Number Synonyms Comment Organism
4.2.3.108 CinS
-
 Lavandula x intermedia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.108 0.0088
-
 geranyl diphosphate pH 7.0, 30°C Lavandula x intermedia

Expression

EC Number Organism Comment Expression
4.2.3.108 Lavandula x intermedia LiCINSmRNAlevels parallel the 1,8-cineole content in mature flowers and in developmental stages additional information