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Literature summary extracted from

  • Crocoll, C.; Asbach, J.; Novak, J.; Gershenzon, J.; Degenhardt, J.
    Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis (2010), Plant Mol. Biol., 73, 587-603.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.100 expressed in Escherichia coli BL21(DE3) cells Origanum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.114 0.00871
-
 geranyl diphosphate pH 6.8, 30°C Origanum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.100 Mg2+ in the presence of Mg2+, the enzyme produces bicyclogermacrene and alloaromadendrene as main products Origanum vulgare
4.2.3.100 Mn2+ in the presence of Mn2+, the enzyme produces mainly bicyclogermacrene Origanum vulgare
4.2.3.114 Mg2+ or Mn2+, required. Optimum concentration of Mg2+ 0.025 mM, 44% of the activity wit Mn2+ Origanum vulgare
4.2.3.114 Mn2+ or Mg2+, required. Mn2+ is preferred over Mg2+, optimum concentration 0.001 mM Origanum vulgare

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.100 Origanum vulgare
-
-
-
4.2.3.114 Origanum vulgare E2E2P0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.100 leaf
-
 Origanum vulgare
-
4.2.3.100 trichome peltate glandular trichome Origanum vulgare
-
4.2.3.114 leaf
-
 Origanum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.100 (2E,6E)-farnesyl diphosphate
-
 Origanum vulgare bicyclogermacrene + diphosphate bicyclogermacrene is the major product in the presence of Mn2+, minor products are (E)-beta-caryophyllene, alpha-humulene, alloaromadendrene, and an unknown sesquiterpene ?
4.2.3.100 additional information geraniol is the main product from geranyl diphosphate in the presence of either Mn2+ or Mg2+, minor products are myrcene, limonene, gamma-terpinene, cis-ocimene, trans-beta-ocimene, terpinolene, linalool, and alpha-terpineol Origanum vulgare ?
-
 ?
4.2.3.114 geranyl diphosphate
-
 Origanum vulgare gamma-terpinene + diphosphate products are 80% gamma-terpinene and the minor products, alpha-thujene (7.2%), alpha-terpinene (6%), myrcene (2.6%), sabinene (1.3%), (+)-R-limonene (1.2%), alpha-pinene (1%) and alpha-phellandrene (0.8%), as well as trace amounts of p-cymene and beta-pinene ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.100 TPS4
-
 Origanum vulgare
4.2.3.114 RPS2
-
 Origanum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.114 28
-
-
 Origanum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.114 6.8
-
-
 Origanum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.114 6 6.5 half maximal activity Origanum vulgare
4.2.3.114 7.2 7.5 half maximal activity Origanum vulgare

Expression

EC Number Organism Comment Expression
4.2.3.114 Origanum vulgare terpene synthase expression levels directly control the composition of the essential oil additional information