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Literature summary extracted from

  • Kampranis, S.C.; Ioannidis, D.; Purvis, A.; Mahrez, W.; Ninga, E.; Katerelos, N.A.; Anssour, S.; Dunwell, J.M.; Degenhardt, J.; Makris, A.M.; Goodenough, P.W.; Johnson, C.B.
    Rational conversion of substrate and product specificity in a Salvia monoterpene synthase: structural insights into the evolution of terpene synthase function (2007), Plant Cell, 19, 1994-2005.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.108 to 1.95 A resolution, space group C2221. alpha-Helical protein comprised of 23 alpha-helices and eight 3/10 helices Salvia fruticosa

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.108 additional information conversion of CinS1 to a sabinene synthase based on an amino acid comparison of the enzyme with Salvia officinalis SabS1 and Salvia pomifera SabS1 Salvia fruticosa
4.2.3.108 N338A products are 11.7% alpha-pinene, 33.1% sabinene, 33.2% beta-pinene, 16.9% myrcene, 5.1% limonene Salvia fruticosa
4.2.3.108 N338C products are 16.1% alpha-pinene, 41% sabinene, 32.1% beta-pinene, 4.7% myrcene, 6.1% limonene Salvia fruticosa
4.2.3.108 N338I products are 6.5% alpha-pinene, 48.3% sabinene, 8.2% myrcene, 37% limonene Salvia fruticosa
4.2.3.108 N338I/A339T products are 5.4% alpha-pinene, 62.1% sabinene, 6.4% myrcene, 26.1% limonene Salvia fruticosa
4.2.3.108 N338I/A339T/G447S products are 5.7% alpha-pinene, 59.6% sabinene, 5.0% myrcene, 29.7% limonene Salvia fruticosa
4.2.3.108 N338I/A339T/G447S/I449P/P450T products are 86.8% sabinene, 5.5% myrcene, 7.7% limonene Salvia fruticosa
4.2.3.108 N338L products are 4.7% alpha-pinene, 5.6% sabinene, 13.2% beta-pinene, 27.2% myrcene, 49.3% limonene Salvia fruticosa
4.2.3.108 N338S products are 9.8% alpha-pinene, 34.5% sabinene, 34.2% beta-pinene, 6.8% myrcene, 2.6% limonene, 5.6% alpha-cineole, 6.5% alpha-terpineol Salvia fruticosa
4.2.3.108 N338V products are 61.2% sabinene, 8.0% myrcene, 30.8% limonene Salvia fruticosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.108 0.0119
-
 geranyl diphosphate mutant N338A, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.0129
-
 geranyl diphosphate mutant N338I/A339T/G447S/I449P/P450T, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.0144
-
 geranyl diphosphate mutant N338S, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.0265
-
 geranyl diphosphate mutant N338L, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.055
-
 geranyl diphosphate mutant N338V, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.0566
-
 geranyl diphosphate mutant N338I, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.0654
-
 geranyl diphosphate wild-type, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.0955
-
 geranyl diphosphate mutant N338I/A339T/G447S, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.136
-
 geranyl diphosphate mutant N338I/A339T, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.201
-
 geranyl diphosphate mutant N338C, pH 7.0, 22°C Salvia fruticosa

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.108 Salvia fruticosa A6XH05
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.108 geranyl diphosphate + H2O
-
 Salvia fruticosa 1,8-cineole + diphosphate 72.4% 1,8-cineole, 7.1% alpha-terpineol, 9.1% beta-pinene, 4.6% alpha-pinene, 3.6% sabinene, 2.2% myrcene, and <1% limonene ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.108 SabS1
-
 Salvia fruticosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.108 0.0043
-
 geranyl diphosphate mutant N338I/A339T/G447S, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.0043
-
 geranyl diphosphate mutant N338I/A339T/G447S/I449P/P450T, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.009
-
 geranyl diphosphate mutant N338L, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.012
-
 geranyl diphosphate mutant N338V, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.022
-
 geranyl diphosphate mutant N338S, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.024
-
 geranyl diphosphate mutant N338I/A339T, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.052
-
 geranyl diphosphate mutant N338C, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.053
-
 geranyl diphosphate wild-type, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.069
-
 geranyl diphosphate mutant N338A, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.096
-
 geranyl diphosphate mutant N338I, pH 7.0, 22°C Salvia fruticosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.108 0.045
-
 geranyl diphosphate mutant N338I/A339T/G447S, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.177
-
 geranyl diphosphate mutant N338I/A339T, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.22
-
 geranyl diphosphate mutant N338V, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.26
-
 geranyl diphosphate mutant N338C, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.33
-
 geranyl diphosphate mutant N338I/A339T/G447S/I449P/P450T, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.34
-
 geranyl diphosphate mutant N338L, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 0.81
-
 geranyl diphosphate wild-type, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 1.53
-
 geranyl diphosphate mutant N338S, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 1.69
-
 geranyl diphosphate mutant N338I, pH 7.0, 22°C Salvia fruticosa
4.2.3.108 5.79
-
 geranyl diphosphate mutant N338A, pH 7.0, 22°C Salvia fruticosa