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Literature summary extracted from
- A geraniol-synthase gene from Cinnamomum tenuipilum (2005), Phytochemistry, 66, 285-293.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.7.11 | DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Cinnamomum tenuipile |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.7.11 | 0.0558 | - |
geranyl diphosphate | pH 7.0, 30°C | Cinnamomum tenuipile |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.7.11 | Mg2+ | dependent on, optimal at 10 mM | Cinnamomum tenuipile | |
| 3.1.7.11 | Mn2+ | dependent on, optimal at 0.5 mM | Cinnamomum tenuipile | |
| 3.1.7.11 | additional information | no effect on enzyme activity by K+ | Cinnamomum tenuipile |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.7.11 | 69000 | - |
x * 69000, sequence calculation | Cinnamomum tenuipile |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.7.11 | geranyl diphosphate + H2O | Cinnamomum tenuipile | - |
geraniol + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.7.11 | Cinnamomum tenuipile | Q8GUE4 | gene CtGES | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.7.11 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Cinnamomum tenuipile |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.7.11 | leaf | CtGES mRNA is localized in the oil cells of the leaves | Cinnamomum tenuipile | - |
| 3.1.7.11 | plant oil gland | CtGES mRNA is localized in the oil cells of the leaves | Cinnamomum tenuipile | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.7.11 | geranyl diphosphate + H2O | - |
Cinnamomum tenuipile | geraniol + diphosphate | - |
? | |
| 3.1.7.11 | geranyl diphosphate + H2O | the monoterpene synthase specifically converts geranyl diphosphate to geraniol, GC-MS analysis | Cinnamomum tenuipile | geraniol + diphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.7.11 | ? | x * 69000, sequence calculation | Cinnamomum tenuipile |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.7.11 | geraniol-synthase | - |
Cinnamomum tenuipile |
| 3.1.7.11 | monoterpene synthase | - |
Cinnamomum tenuipile |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.7.11 | 30 | - |
assay at | Cinnamomum tenuipile |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.7.11 | 7 | - |
- |
Cinnamomum tenuipile |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.7.11 | 6.2 | 8.2 | 20% of maximal activity at pH 6.2 and pH 8.2 | Cinnamomum tenuipile |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.7.11 | Cinnamomum tenuipile | sequence calculation | - |
5.67 |
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