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Literature summary extracted from

  • Teufel, R.; Friedrich, T.; Fuchs, G.
    An oxygenase that forms and deoxygenates toxic epoxide (2012), Nature, 483, 359-362.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.149 expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.149 benzoyl-CoA
-
Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.149 0.003
-
O2 at pH 8.0 and 30°C Pseudomonas sp.
1.14.13.149 0.006
-
epoxyphenylacetyl-CoA at pH 8.0 and 30°C Pseudomonas sp.
1.14.13.149 0.023
-
NADPH at pH 8.0 and 30°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.149 Fe2+ contains a catalytic di-iron center Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.149 Pseudomonas sp.
-
-
-
1.14.13.149 Pseudomonas sp. Y2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.149 amylose resin column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.149 epoxyphenylacetyl-CoA + O2 + NADPH + H+ the di-iron center of PaaABCE also deoxygenates epoxyphenylacetyl-CoA, which occurs when phenylacetyl-CoA is largely used up Pseudomonas sp. ?
-
?
1.14.13.149 epoxyphenylacetyl-CoA + O2 + NADPH + H+ the di-iron center of PaaABCE also deoxygenates epoxyphenylacetyl-CoA, which occurs when phenylacetyl-CoA is largely used up Pseudomonas sp. Y2 ?
-
?
1.14.13.149 additional information he non-physiological substrate benzoyl-CoA is not transformed to detectable products Pseudomonas sp. ?
-
?
1.14.13.149 additional information he non-physiological substrate benzoyl-CoA is not transformed to detectable products Pseudomonas sp. Y2 ?
-
?
1.14.13.149 phenylacetyl-CoA + O2 + NADPH + H+
-
Pseudomonas sp. 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+
-
?
1.14.13.149 phenylacetyl-CoA + O2 + NADPH + H+
-
Pseudomonas sp. Y2 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.149 PaaABCE
-
Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.149 8
-
-
Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.149 FAD
-
Pseudomonas sp.
1.14.13.149 NADPH
-
Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.14.13.149 metabolism bifunctional PaaABCE acts as a phenylacetyl-CoA ring-1,2-epoxidase/ring-1,2-epoxyphenylacetyl-CoA deoxygenase Pseudomonas sp.