EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.149 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.149 | benzoyl-CoA | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.149 | 0.003 | - |
O2 | at pH 8.0 and 30°C | Pseudomonas sp. | |
1.14.13.149 | 0.006 | - |
epoxyphenylacetyl-CoA | at pH 8.0 and 30°C | Pseudomonas sp. | |
1.14.13.149 | 0.023 | - |
NADPH | at pH 8.0 and 30°C | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.149 | Fe2+ | contains a catalytic di-iron center | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.149 | Pseudomonas sp. | - |
- |
- |
1.14.13.149 | Pseudomonas sp. Y2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.149 | amylose resin column chromatography | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.149 | epoxyphenylacetyl-CoA + O2 + NADPH + H+ | the di-iron center of PaaABCE also deoxygenates epoxyphenylacetyl-CoA, which occurs when phenylacetyl-CoA is largely used up | Pseudomonas sp. | ? | - |
? | |
1.14.13.149 | epoxyphenylacetyl-CoA + O2 + NADPH + H+ | the di-iron center of PaaABCE also deoxygenates epoxyphenylacetyl-CoA, which occurs when phenylacetyl-CoA is largely used up | Pseudomonas sp. Y2 | ? | - |
? | |
1.14.13.149 | additional information | he non-physiological substrate benzoyl-CoA is not transformed to detectable products | Pseudomonas sp. | ? | - |
? | |
1.14.13.149 | additional information | he non-physiological substrate benzoyl-CoA is not transformed to detectable products | Pseudomonas sp. Y2 | ? | - |
? | |
1.14.13.149 | phenylacetyl-CoA + O2 + NADPH + H+ | - |
Pseudomonas sp. | 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+ | - |
? | |
1.14.13.149 | phenylacetyl-CoA + O2 + NADPH + H+ | - |
Pseudomonas sp. Y2 | 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.149 | PaaABCE | - |
Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.149 | 8 | - |
- |
Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.149 | FAD | - |
Pseudomonas sp. | |
1.14.13.149 | NADPH | - |
Pseudomonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.149 | metabolism | bifunctional PaaABCE acts as a phenylacetyl-CoA ring-1,2-epoxidase/ring-1,2-epoxyphenylacetyl-CoA deoxygenase | Pseudomonas sp. |