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Literature summary extracted from

  • Wang, S.L.; Liang, T.W.; Lin, B.S.; Wang, C.L.; Wu, P.C.; Liu, J.R.
    Purification and characterization of chitinase from a new species strain Pseudomonas sp. TKU008 (2010), J. Microbiol. Biotechnol., 20, 1001-1005.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Cu2+ complete inhibition at 5 mM Pseudomonas sp.
3.2.1.14 Mn2+ complete inhibition at 5 mM Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 40000
-
 x * 40000, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Pseudomonas sp.
-
 isolated from soil in Taiwan
-
3.2.1.14 Pseudomonas sp. TKU008
-
 isolated from soil in Taiwan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme 25fold by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 cell culture culture condition suitable for production of chitinase are shaking at 30°C for 4 days in 100 ml of medium containing 1% shrimp and crab shell powder, 0.1% K2HPO4, and 0.05% MgSO4·7H2O, pH 7.0 Pseudomonas sp.
-
3.2.1.14 additional information TKU008 chitinase is suppressed by the simultaneously existing protease, which also showed the maximum activity at the fourth day of incubation Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 0.175
-
 purified enzyme, pH 7.0, 37°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 colloidal chitin + H2O
-
 Pseudomonas sp. ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Pseudomonas sp. TKU008 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 40000, SDS-PAGE Pseudomonas sp.
3.2.1.14 More peptide mass mapping, overview Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 50
-
 stable up to Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 6 7 stable Pseudomonas sp.