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Literature summary extracted from
- Isopeptide ligation catalyzed by quintessential sortase A: mechanistic cues from cyclic and branched oligomers of indolicidin (2011), J. Biol. Chem., 286, 23996-24006.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.22.70 | expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.70 | 1.18 | - |
agmatine | at 37°C, pH not specified in the publication, transpeptidation reaction | Staphylococcus aureus |  |
| 3.4.22.70 | 1.59 | - |
YALPETGK | at 37°C, pH not specified in the publication | Staphylococcus aureus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.70 | Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.70 | Ni-NTA column chromatography | Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.70 | agmatine + YALPETGK | transpeptidase reaction. The initially formed acyl-enzyme intermediate undergoes a hydrolysis followed by intra-molecular transpeptidation | Staphylococcus aureus | (NH2)2-CN-(CH2)4-NH-(CO)-TEPLAY + ? | - |
? | |
| 3.4.22.70 | additional information | sortase A also mediates transpeptidation reaction of indolicidin-derived peptides | Staphylococcus aureus | ? | - |
? | |
| 3.4.22.70 | YALPETGK + H2O | - |
Staphylococcus aureus | YALPET + Gly-Lys | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.22.70 | SrtA | - |
Staphylococcus aureus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.22.70 | physiological function | the enzyme catalyzes the covalent anchoring of surface proteins to the cell wall by linking the threonyl carboxylate of the LPXTG recognition motif to the amino group of the pentaglycine cross-bridge of the peptidoglycan | Staphylococcus aureus |
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