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Literature summary extracted from

  • Kim, K.S.; Pelton, J.G.; Inwood, W.B.; Andersen, U.; Kustu, S.; Wemmer, D.E.
    The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems (2010), J. Bacteriol., 192, 4089-4102.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.46 gene rutA, recombinant overexpression of His-tagged RutA in ASKA strain JW0997 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.110 additional information construction of diverse rutB-deficient strains, overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.46 thymine + FMNH2 + O2 Escherichia coli
-
 (Z)-2-methylureidoacrylate peracid + FMN
-
 ?
1.14.99.46 uracil + FMNH2 + O2 Escherichia coli
-
 (Z)-3-ureidoacrylate peracid + FMN
-
 ?
3.5.1.110 ureidoacrylate peracid + H2O Escherichia coli
-
 peroxyaminoacrylate + NH3 + CO2
-
 ?
3.5.1.110 ureidoacrylate peracid + H2O Escherichia coli JW5139
-
 peroxyaminoacrylate + NH3 + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.46 Escherichia coli P75898 gene rutA
-
3.5.1.110 Escherichia coli P75897 K-12, gene rutB
-
3.5.1.110 Escherichia coli JW5139 P75897 K-12, gene rutB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.46 recombinant His-tagged RutA from ASKA strain JW0997 by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.46 additional information (Z)-3-ureidoacrylic peracid is not stable and decomposes nonenzymatically to give (Z)-3-ureidoacrylate Escherichia coli ?
-
 ?
1.14.99.46 thymine + FMNH2 + O2
-
 Escherichia coli (Z)-2-methylureidoacrylate peracid + FMN
-
 ?
1.14.99.46 uracil + FMNH2 + O2
-
 Escherichia coli (Z)-3-ureidoacrylate peracid + FMN
-
 ?
1.14.99.46 uracil + FMNH2 + O2 RutA directly cleaves the uracil ring between N-3 and C-4 to yield ureidoacrylate Escherichia coli (Z)-3-ureidoacrylate peracid + FMN product analysis by both NMR spectroscopy and mass spectrometry, overview ?
3.5.1.110 additional information in vitro RutB cleaves ureidoacrylate hydrolytically to release 2 mol of ammonium, malonic semialdehyde, and carbon dioxide Escherichia coli ?
-
 ?
3.5.1.110 additional information in vitro RutB cleaves ureidoacrylate hydrolytically to release 2 mol of ammonium, malonic semialdehyde, and carbon dioxide Escherichia coli JW5139 ?
-
 ?
3.5.1.110 ureidoacrylate peracid + H2O
-
 Escherichia coli peroxyaminoacrylate + NH3 + CO2
-
 ?
3.5.1.110 ureidoacrylate peracid + H2O
-
 Escherichia coli JW5139 peroxyaminoacrylate + NH3 + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.46 RutA
-
 Escherichia coli
3.5.1.110 RutB
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.46 22
-
 assay at room temperature Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.46 7 8.2 assay at Escherichia coli
3.5.1.110 8.2
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.46 FAD FAD works much less well than FMN Escherichia coli
1.14.99.46 FMN preferred cofactor Escherichia coli

General Information

EC Number General Information Comment Organism
1.14.99.46 metabolism RutA has a central role in the Rut pathway, a pyrimidine catabolic pathway, which is composed of seven proteins, all of which are required by Escherichia coli K-12 to grow on uracil as the sole nitrogen source, pathway overview. RutA works in conjunction with the flavin reductase RutF Escherichia coli
1.14.99.46 physiological function RutA has a central role in the Rut pathway, which is composed of seven proteins, all of which are required by Escherichia coli K-12 to grow on uracil as the sole nitrogen source Escherichia coli
3.5.1.110 metabolism functions of enzymes encoded in the rut gene cluster, overview Escherichia coli