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Literature summary extracted from
- Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600 (1996), J. Bacteriol., 178, 6123-6132.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.10 | expressed in Escherichia coli DH5alpha cells | Aminobacter aminovorans |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.10 | Mg2+ | required | Aminobacter aminovorans |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.10 | 50500 | - |
x * 50500, calculated from amino acid sequence | Aminobacter aminovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.10 | Aminobacter aminovorans | P54989 | component A of NTA monooxygenase | - |
| 1.14.14.10 | Aminobacter aminovorans ATCC 29600 | P54989 | component A of NTA monooxygenase | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.10 | 0.076 | - |
in 30 mM Tris-HCl (pH 8.0), 10 mM FMN, and 2 mM MgCl2, temperature not specified in the publication | Aminobacter aminovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.10 | nitrilotriacetate + FMNH2 + H+ + O2 | - |
Aminobacter aminovorans | iminodiacetate + glyoxylate + FMN + H2O | - |
ir | |
| 1.14.14.10 | nitrilotriacetate + FMNH2 + H+ + O2 | - |
Aminobacter aminovorans ATCC 29600 | iminodiacetate + glyoxylate + FMN + H2O | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.10 | ? | x * 50500, calculated from amino acid sequence | Aminobacter aminovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.10 | NTA monooxygenase | - |
Aminobacter aminovorans |
| 1.14.14.10 | NTA-MO | - |
Aminobacter aminovorans |
| 1.14.14.10 | NtaA | component A of NTA monooxygenase | Aminobacter aminovorans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.10 | FMNH2 | - |
Aminobacter aminovorans | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.14.10 | Aminobacter aminovorans | calculated from amino acid sequence | - |
6.5 |
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