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Literature summary extracted from

  • Maupin-Furlow, J.; Ferry, J.
    Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron-sulfur enzyme of the CO dehydrogenase complex from Methanosarcina thermophila (1996), J. Bacteriol., 178, 340-346.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.245 genes cdhE and cdhD, the cdhD gene is located upstream of cdhE, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.245 Co2+ CdhE contains approximately 4.5 mol of Fe, 2.7 mol of acid-labile sulfur, and 0.3 mol of hydroxocobalamin per mol of purified CdhE Methanosarcina thermophila
2.1.1.245 Fe2+ CdhE contains approximately 4.5 mol of Fe, 2.7 mol of acid-labile sulfur, and 0.3 mol of hydroxocobalamin per mol of purified CdhE Methanosarcina thermophila
2.1.1.245 Fe2+ in the iron-sulfur protein Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.245 Methanosarcina thermophila Q50538 CdhD1; genes cdhD and cdhE
-
2.1.1.245 Methanosarcina thermophila Q50539 CdhE1; genes cdhD and cdhE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.245 recombinant CdhD from Escherichia coli strain BL21(DE3) Methanosarcina thermophila
2.1.1.245 recombinant CdhE from Escherichia coli strain BL21(DE3) Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.245 additional information reduction of the cobalt atom of the corrinoid to Co1+ by Ti(III)citrate, the gamma subunit CdhE contains the single 4Fe-4S center in the C/Fe-S enzyme Methanosarcina thermophila ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.245 More the C/Fe-S enzyme purified contains one molecule of factor III in the base-off form which facilitates reduction of the cobalt atom to the Co1+ redox state, which is a requirement for methylation Methanosarcina thermophila

Synonyms

EC Number Synonyms Comment Organism
2.1.1.245 C/Fe-S enzyme
-
 Methanosarcina thermophila
2.1.1.245 CdhD1
-
 Methanosarcina thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.245 13
-
 assay at Methanosarcina thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.245 7.6
-
 assay at Methanosarcina thermophila

General Information

EC Number General Information Comment Organism
2.1.1.245 additional information genes cdhD and cdhE encode the delta and gamma subunits of the C/Fe-S enzyme from the CO dehydrogenase complex of Methanosarcina thermophila, the complex comprises a nickel/iron-sulfur, Ni/Fe-S, enzyme containing alpha and epsilon subunits and a corrinoid/iron-sulfur, C/Fe-S, enzyme containing gamma and delta subunits. The C/Fe-S enzyme contains one molecule of factor III in the base-off form which facilitates reduction of the cobalt atom to the Co1+ redox state, which is a requirement for methylation. The gamma subunit CdhE contains the single 4Fe-4S center in the C/Fe-S enzyme, overview Methanosarcina thermophila
2.1.1.245 additional information genes cdhD and cdhE encode the delta and gamma subunits of the C/Fe-S enzyme from the CO dehydrogenase complex of Methanosarcina thermophila, the complex comprises a nickel/iron-sulfur, Ni/Fe-S, enzyme containing alpha and epsilon subunits and a corrinoid/iron-sulfur, C/Fe-S, enzyme containing gamma and delta subunits. The C/Fe-S enzyme purified contains one molecule of factor III in the base-off form which facilitates reduction of the cobalt atom to the Co1+ redox state, which is a requirement for methylation. The gamma subunit CdhE contains the single 4Fe-4S center in the C/Fe-S enzyme Methanosarcina thermophila