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Literature summary extracted from

  • Ropp, J.; Gunsalus, I.; Sligar, S.
    Cloning and expression of a member of a new cytochrome P-450 family: Cytochrome P-450lin (CYP111) from Pseudomonas incognita (1993), J. Bacteriol., 175, 6028-6037.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.84 gene linC, DNA and amino acid sequence determination and analysis, constitutive functional expression in Escherichia coli from plasmid pUC18 under control of the lac promoter Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.84 Fe2+ heme-containing cytochrome P450 enzyme Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Pseudomonas putida
-
 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Pseudomonas putida PpG777
-
 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.84 Pseudomonas putida Q59723 a D110R strain, gene linC
-
1.14.14.84 Pseudomonas putida PpG777 Q59723 a D110R strain, gene linC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.84 recombinant enzyme from Escherichia coli by anion exchange chromatography, ammonium sulfate fractionation, gel filtration, and a second step of anion exchange chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Pseudomonas putida (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Pseudomonas putida PpG777 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.84 P45O1in
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.84 cytochrome P450
-
 Pseudomonas putida
1.14.14.84 heme
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.14.14.84 evolution P45O1in is a member of the CYPIll P-450 monooxygenase gene family Pseudomonas putida
1.14.14.84 metabolism P-4501in catalyzes the 8-methyl hydroxylation of linalool as the first committed step of its utilization by Pseudomonas putida as the sole carbon source Pseudomonas putida
1.14.14.84 additional information P-4501in exhibits conservation in the axial cysteine heme ligand-containing peptide and the threonine region postulated to form an 02-binding pocket Pseudomonas putida