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Literature summary extracted from

  • Riaz, M.; Rashid, M.; Sawyer, L.; Akhtar, S.; Javed, M.; Nadeem, H.; Wear, M.
    Physiochemical properties and kinetics of glucoamylase produced from deoxy-D-glucose resistant mutant of Aspergillus niger for soluble starch hydrolysis (2012), Food Chem., 130, 24-30.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information thermodynamics and kinetics of starch hydrolysis, overview Aspergillus niger
3.2.1.3 0.16
-
 starch mutant enzyme, pH 5.0, 40°C Aspergillus niger
3.2.1.3 0.25
-
 starch wild-type enzyme, pH 5.0, 40°C Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 72063
-
 1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry Aspergillus niger
3.2.1.3 72876
-
 1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry Aspergillus niger
3.2.1.3 93000
-
 1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus niger
-
 locally isolated Aspergillus niger, wild-type and deoxy-D-glucose-resistant mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 glucoamylases from a wild-type and a deoxy-D-glucose-resistant mutant Aspergillus niger to apparent homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography, the wild-tyype enzyme is purified 25.4fold, the mutant 30.6fold Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 216
-
 purified wild-type enzyme, pH 5.0, 40°C, substrate starch Aspergillus niger
3.2.1.3 495.2
-
 purified mutant enzyme, pH 5.0, 40°C, substrate starch Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O
-
 Aspergillus niger beta-D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 monomer 1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 40
-
 assay at Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 25 70 activity range Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 343
-
 starch wild-type enzyme, pH 5.0, 40°C Aspergillus niger
3.2.1.3 727
-
 starch mutant enzyme, pH 5.0, 40°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5
-
 assay at Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 3 6 activity range Aspergillus niger

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.3 1347
-
 starch wild-type enzyme, pH 5.0, 40°C Aspergillus niger
3.2.1.3 4510
-
 starch mutant enzyme, pH 5.0, 40°C Aspergillus niger