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Literature summary extracted from

  • Li, L.; Arnosti, D.
    Fine mapping of chromatin structure in Drosophila melanogaster embryos using micrococcal nuclease (2010), Fly, 4, 213-215.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.31.1 analysis the enzyme is a useful tool for mapping chromatin structure in eukaryotes, usage of the enzyme to determine the positions of nucleosomes within a region of DNA to identify dynamic changes induced during gene regulation Staphylococcus aureus
3.1.31.1 molecular biology the enzyme is a useful tool for mapping chromatin structure in eukaryotes, usage of the enzyme to determine the positions of nucleosomes within a region of DNA to identify dynamic changes induced during gene regulation Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.31.1 additional information Staphylococcus aureus micrococcal nuclease induces double-strand breaks within nucleosome linker regions, and with more extensive digestion, single-strand nicks within the nucleosome itself ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.31.1 Staphylococcus aureus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.31.1 embryo
-
 Staphylococcus aureus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.31.1 additional information micrococcal nuclease induces double-strand breaks within nucleosome linker regions, and with more extensive digestion, single-strand nicks within the nucleosome itself Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.31.1 Micrococcal nuclease
-
 Staphylococcus aureus
3.1.31.1 MN ase
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.31.1 37
-
 assay at Staphylococcus aureus

General Information

EC Number General Information Comment Organism
3.1.31.1 additional information MNase mapping of the enhancer chromatin structure in the Drosophila melanogaster embryo to analyse chromatin structure in a developmental setting, and identification of structural changes on a cis-regulatory element targeted by the Knirps repressor, method development and optimization, overview Staphylococcus aureus