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Literature summary extracted from

  • Wu, Y.; Hillwig, M.L.; Wang, Q.; Peters, R.J.
    Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7 (2011), FEBS Lett., 585, 3446-3451.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.69 expression in Escherichia coli Oryza sativa Japonica Group
1.14.14.76 expressed in Escherichia coli C41 cells Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.69 0.2
-
 ent-cassa-12,15-diene pH not specified in the publication, temperature not specified in the publication Oryza sativa Japonica Group
1.14.14.76 0.012
-
 ent-isokaurene pH and temperature not specified in the publication Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.69 Oryza sativa Japonica Group Q6YV88
-
-
1.14.14.76 Oryza sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.69 ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
 Oryza sativa Japonica Group ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.76 ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa ent-2alpha-hydroxyisokaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
1.14.14.76 additional information the enzyme does not react with ent-cassadiene Oryza sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.76 CYP71Z6
-
 Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.69 0.11
-
 ent-cassa-12,15-diene pH not specified in the publication, temperature not specified in the publication Oryza sativa Japonica Group
1.14.14.76 0.01
-
 ent-isokaurene pH and temperature not specified in the publication Oryza sativa

General Information

EC Number General Information Comment Organism
1.14.14.76 metabolism the enzyme acts as a C2-hydroxylase involved in a latter step of phytocassane biosynthesis Oryza sativa