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Literature summary extracted from

  • Xun, L.; Reeder, R.; Plymale, A.; Girvin, D.; Bolton Jr., H.
    Degradation of metal - nitrilotriacetate complexes by nitrilotriacetate monooxygenase (1996), Environ. Sci. Technol., 30, 1752-1755.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.10 Ni2+ ionic or uncomplexed Ni2+ (2 mM) is inhibitory to the enzyme Aminobacter aminovorans
1.14.14.10 Zn2+ ionic or uncomplexed Zn2+ (2 mM) is inhibitory to the enzyme Aminobacter aminovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.10 0.0317
-
 nitrilotriacetate with equimolar Ni2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
1.14.14.10 0.0367
-
 nitrilotriacetate with equimolar Co2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
1.14.14.10 0.0404
-
 nitrilotriacetate with equimolar Mg2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
1.14.14.10 0.043
-
 nitrilotriacetate with 2 mM Mg2+and varying nitrilotriacetate concentrations, in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
1.14.14.10 0.0576
-
 nitrilotriacetate with equimolar Zn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
1.14.14.10 0.0905
-
 nitrilotriacetate with equimolar Mn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
1.14.14.10 0.0968
-
 nitrilotriacetate with equimolar Fe2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.10 Al3+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
1.14.14.10 Co2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
1.14.14.10 Cu2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
1.14.14.10 Fe2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
1.14.14.10 Mg2+ the enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate Aminobacter aminovorans
1.14.14.10 Ni2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
1.14.14.10 Zn2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.10 Aminobacter aminovorans
-
-
-
1.14.14.10 Aminobacter aminovorans ATCC 29600
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.10 partially purified by ammonium sulfate precipitation and phenyl agarose column chromatography Aminobacter aminovorans

Storage Stability

EC Number Storage Stability Organism
1.14.14.10 -80°C, partially purified, dialyzed enzyme, 3 months, no loss of activity Aminobacter aminovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.10 additional information nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate Aminobacter aminovorans ?
-
 ?
1.14.14.10 additional information nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate Aminobacter aminovorans ATCC 29600 ?
-
 ?
1.14.14.10 nitrilotriacetate + FMNH2 + H+ + O2 nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate Aminobacter aminovorans iminodiacetate + glyoxylate + FMN + H2O
-
 ?
1.14.14.10 nitrilotriacetate + FMNH2 + H+ + O2 nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate Aminobacter aminovorans ATCC 29600 iminodiacetate + glyoxylate + FMN + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.10 NTA-MO
-
 Aminobacter aminovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.10 FMNH2
-
 Aminobacter aminovorans