Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yu, F.; Okamoto, S.; Harada, H.; Yamasaki, K.; Misawa, N.; Utsumi, R.
    Zingiber zerumbet CYP71BA1 catalyzes the conversion of ?-humulene to 8-hydroxy-alpha-humulene in zerumbone biosynthesis (2011), Cell. Mol. Life Sci., 68, 1033-1040.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.113 functional co-expression of CYP71BA1 with Arabidopsis NADPH-dependent P450 reductase in Saccharomyces cerevisiae strain WAT11 microsomes, additional cloning of Arabidopsis thaliana ATR2 and Zingiber zerumbet ZSS1 encoding an alpha-humulene synthase Zingiber zerumbet
4.2.3.104 expressed in Escherichia coli BL21(DE3) cells Zingiber zerumbet

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.113 additional information metabolic engineering: functional co-expression of a gene cluster encoding four enzymes of the mevalonate pathway, i.e. mevalonate kinase, phosphomevalonate kinase, mevalonate diphosphate decarboxylase, and isopentenyl diphosphate isomerase, with CYP71BA1 and ZSS1, encoding alpha-humulene synthase, in Escherichia coli leading to the production of 10-hydroxy-alpha-humulene in the presence of mevalonate, overview Zingiber zerumbet

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.113 Fe2+ cytochrome P450 enzyme Zingiber zerumbet

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.113 alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase] Zingiber zerumbet
-
 10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.113 Zingiber zerumbet E3W9C4
-
-
4.2.3.104 Zingiber zerumbet
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.113 rhizome CYP71BA1 transcripts are detected almost exclusively in rhizomes, expression pattern of ZSS1 transcripts during rhizome development Zingiber zerumbet
-
4.2.3.104 leaf
-
 Zingiber zerumbet
-
4.2.3.104 additional information not detected in stem Zingiber zerumbet
-
4.2.3.104 rhizome almost exclusively expressed in rhizomes Zingiber zerumbet
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.113 alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
-
 Zingiber zerumbet 10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.113 alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase] CYP71BA1 is a regiospecific cytochrome P450 enzyme Zingiber zerumbet 10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O GC-MS product analysis ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.113 CYP71BA1
-
 Zingiber zerumbet
4.2.3.104 alpha-humulene synthase
-
 Zingiber zerumbet
4.2.3.104 ZSS1
-
 Zingiber zerumbet

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.113 30
-
 assay at Zingiber zerumbet

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.113 8
-
 assay at Zingiber zerumbet

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.113 cytochrome P450
-
 Zingiber zerumbet

Expression

EC Number Organism Comment Expression
4.2.3.104 Zingiber zerumbet the transcript of ZZS1 gene increases very rapidly from June 12 to June 28, a period of strong temperature increase in 2007. Transcript level remains high throughout the summer and autumn, but declines significantly in December up

General Information

EC Number General Information Comment Organism
1.14.14.113 evolution the enzyme belongs to the cytochrome P450 monooxygenases Zingiber zerumbet