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Literature summary extracted from

  • Kanudia, P.; Mittal, M.; Kumaran, S.; Chakraborti, P.
    Amino-terminal extension present in the methionine aminopeptidase type 1c of Mycobacterium tuberculosis is indispensible for its activity (2011), BMC Biochem., 12, 35.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.18 expressed in Escherichia coli BL21(DE3) Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.18 D117A lining the perimeter of the active site, no activity Mycobacterium tuberculosis
3.4.11.18 D131A no activity Mycobacterium tuberculosis
3.4.11.18 DELTA 2-10 30% activity with 10fold amount of enzyme in the assay relative to wild type Mycobacterium tuberculosis
3.4.11.18 DELTA 2-15 30% activity with 10fold amount of enzyme in the assay relative to wild type Mycobacterium tuberculosis
3.4.11.18 DELTA 2-20 no activity Mycobacterium tuberculosis
3.4.11.18 DELTA 2-30 no activity Mycobacterium tuberculosis
3.4.11.18 DELTA 2-40 no activity Mycobacterium tuberculosis
3.4.11.18 E219V predicted active site residue, no activity Mycobacterium tuberculosis
3.4.11.18 E238A predicted active site residue, no activity Mycobacterium tuberculosis
3.4.11.18 H114A predicted active site residue, no activity Mycobacterium tuberculosis
3.4.11.18 H193A predicted active site residue, no activity Mycobacterium tuberculosis
3.4.11.18 H212A predicted active site residue, no activity Mycobacterium tuberculosis
3.4.11.18 H88A predicted active site residue, no activity Mycobacterium tuberculosis
3.4.11.18 P19A 65% activity relative to wild type Mycobacterium tuberculosis
3.4.11.18 P19G 35% activity relative to wild type Mycobacterium tuberculosis
3.4.11.18 V18A 30% activity relative to wild type Mycobacterium tuberculosis
3.4.11.18 V18A/P19A no activity Mycobacterium tuberculosis
3.4.11.18 V18G no activity Mycobacterium tuberculosis
3.4.11.18 V18G/P19G no activity Mycobacterium tuberculosis
3.4.11.18 W236L no activity Mycobacterium tuberculosis
3.4.11.18 W255L no activity Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.18 amastatin
-
 Mycobacterium tuberculosis
3.4.11.18 EDTA
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.18 1.36
-
 Met-Ala-Ser pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 2.85
-
 Met-Gly-Met-Met pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 3.3
-
 Met-Ala-Ser pH 7.5, 30°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.18 37300
-
 x * 37300, SDS-PAGE Mycobacterium tuberculosis
3.4.11.18 39100
-
 x * 39100, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.18 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.18 immobilized metal ion affinity chromatography (Ni2+) Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.18 Met-Ala-Ser + H2O
-
 Mycobacterium tuberculosis L-methionine + Ala-Ser
-
 ?
3.4.11.18 Met-Gly-Met-Met + H2O
-
 Mycobacterium tuberculosis L-methionine + Gly-Met-Met
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.18 ? x * 37300, SDS-PAGE Mycobacterium tuberculosis
3.4.11.18 ? x * 39100, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.4.11.18 methionine aminopeptidase type 1a
-
 Mycobacterium tuberculosis
3.4.11.18 methionine aminopeptidase type 1c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.18 30 37
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.18 50
-
 85% activity after 10 min at 50°C, Tm: 53,6°C (determination by CD spectroscopy) Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.18 9.63
-
 Met-Gly-Met-Met calculated with 29.5 kDa as molecular mass, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 11.7
-
 Met-Ala-Ser calculated with 29.5 kDa as molecular mass, pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 1516
-
 Met-Ala-Ser calculated with 33.1 kDa as molecular mass, pH 7.5, 30°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.18 3.32
-
 Met-Ala-Ser pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 3.47
-
 Met-Gly-Met-Met pH 7.5, 30°C Mycobacterium tuberculosis
3.4.11.18 1156
-
 Met-Ala-Ser pH 7.5, 30°C Mycobacterium tuberculosis