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Literature summary extracted from

  • Bose, S.; Barnes, C.A.; Petrich, J.W.
    Enhanced stability and activity of cellulase in an ionic liquid and the effect of pretreatment on cellulose hydrolysis (2011), Biotechnol. Bioeng., 109, 434-443.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 additional information pretreatment of cellulose with ionic liquids such as 1-butyl-3-methylimidazolium chloride, 1-methylimidazolium chloride, and tris-(2-hydroxyethyl)-methylammonium methylsulfate results in more rapid conversion to glucose than hydrolysis with cellulose that is not pretreated Aspergillus niger
3.2.1.4 tris-(2-hydroxyethyl)-methylammonium methylsulfate enzymic activity is significantly enhanced in the presence of tris-(2-hydroxyethyl)-methylammonium methylsulfate in buffer. Tris-(2-hydroxyethyl)-methylammonium methylsulfate imparts substantial thermostability to the enzyme Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 1-butyl-3-methylimidazolium methylsulfate the enzyme precipitates immediately on addition to the reaction mixture Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Aspergillus niger
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 commercial preparation
-
 Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 cellulose + H2O pretreatment of cellulose with ionic liquids such as 1-butyl-3-methylimidazolium chloride, 1-methylimidazolium chloride, and tris-(2-hydroxyethyl)-methylammonium methylsulfate results in more rapid conversion to glucose than hydrolysis with cellulose that is not pretreated Aspergillus niger ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 endo-1,4-beta-D-glucanase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 additional information
-
 the percent conversion to glucose from pretreated cellulose is increased when the temperature is increased from 45 to 60°C Aspergillus niger
3.2.1.4 45
-
-
 Aspergillus niger
3.2.1.4 75
-
 presence of tris-(2-hydroxyethyl)-methylammonium methylsulfate Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 45 75 activity range Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 55
-
 inactivation Aspergillus niger
3.2.1.4 55
-
 denaturation above Aspergillus niger
3.2.1.4 75
-
 denaturation, presence of tris-(2-hydroxyethyl)-methylammonium methylsulfate Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4.8
-
 assay at Aspergillus niger

General Information

EC Number General Information Comment Organism
3.2.1.4 additional information effects of different solvents, ions, and viscosities on enzyme activity at different temperatures, overview Aspergillus niger