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Literature summary extracted from

  • Asano, M.; Nakamura, N.; Kawai, M.; Miwa, T.; Nio, N.
    Purification and characterization of an N-terminal acidic amino acid-specific aminopeptidase from soybean cotyledons (Glycine max) (2010), Biosci. Biotechnol. Biochem., 74, 113-118.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.7 DTT 32% activity at 0.1 mM Glycine max
3.4.11.7 EDTA 83.5% activity at 5 mM Glycine max
3.4.11.7 o-phenanthroline 47.1% activity at 2 mM Glycine max
3.4.11.7 Zn2+ 68.3% activity at 0.4 mM Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.7 56000
-
 x * 56000, SDS-PAGE Glycine max
3.4.11.7 510000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.7 Glycine max
-
 Merril cv. Kegon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.7 ammonium sulfate precipitation, anion exchange chromatography, hydrophobic interaction chromatography, gel filtration Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.7 cotyledon
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.7 66.3
-
 substrate Glu-Glu, pH 7.2, 37°C Glycine max
3.4.11.7 132
-
 substrate Asp-4-nitroanilide, pH 7.2, 37°C Glycine max
3.4.11.7 319
-
 substrate Glu-4-nitroanilide, pH 7.2, 37°C Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.7 Asp-4-nitroanilide + H2O
-
 Glycine max Asp + 4-nitroaniline
-
 ?
3.4.11.7 Glu-4-nitroanilide + H2O
-
 Glycine max Glu + 4-nitroaniline
-
 ?
3.4.11.7 Glu-Glu + H2O many Glu-Xxx and Asp-Xxx dipeptides are substrates Glycine max 2 Glu
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.7 multimer x * 56000, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
3.4.11.7 N-terminal acidic amino acid-specific aminopeptidase
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.7 45
-
 pH 6.0 Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.7 50
-
 stable for 80 min at pH 6.0 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.7 7 9 more than 80% activity at 30°C Glycine max