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Literature summary extracted from

  • Ono, T.; Ohara-Nemoto, Y.; Shimoyama, Y.; Okawara, H.; Kobayakawa, T.; Baba, T.T.; Kimura, S.; Nemoto, T.K.
    Amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases (2010), Biol. Chem., 391, 1221-1232.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.19 Thermolysin
-
Staphylococcus aureus
3.4.21.19 thermolysin v8
-
Staphylococcus saprophyticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.19 expressed in Escherichia coli Staphylococcus cohnii subsp. cohnii
3.4.21.19 expressed in Escherichia coli Staphylococcus caprae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.19 N190H/Y192H/S169A the specific activity of the mutant is 4.5fold increased from that of the wild type enzyme. The mutant potently hydrolyzes LLE-7-amido-4-methylcomarin Staphylococcus epidermidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.19 4-(2-aminoethyl)benzenesulfonyl fluoride
-
Staphylococcus caprae
3.4.21.19 4-(2-aminoethyl)benzenesulfonyl fluoride
-
Staphylococcus cohnii subsp. cohnii
3.4.21.19 diisopropyl fluorophosphate
-
Staphylococcus caprae
3.4.21.19 diisopropyl fluorophosphate
-
Staphylococcus cohnii subsp. cohnii
3.4.21.19 additional information tosyl-L-phenylalanine chloromethyl ketone (0.1 mg/ml), tosyl-L-lysine chloromethyl ketone (50 mg/ml), phenylmethylsulfonyl fluoride (1 mM), aprotinin (0.2 units/ml), leupeptin (0.1 mg/ml), pepstatin (0.01 mg/ml), and dithiothreitol (5 mM) do not inhibit the enzyme activity Staphylococcus caprae
3.4.21.19 additional information tosyl-L-phenylalanine chloromethyl ketone (0.1 mg/ml), tosyl-L-lysine chloromethyl ketone (50 mg/ml), phenylmethylsulfonyl fluoride (1 mM), aprotinin (0.2 units/ml), leupeptin (0.1 mg/ml), pepstatin (0.01 mg/ml), and dithiothreitol (5 mM) do not inhibit the enzyme activity Staphylococcus cohnii subsp. cohnii

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.19 Staphylococcus aureus
-
-
-
3.4.21.19 Staphylococcus aureus ATCC 25923
-
-
-
3.4.21.19 Staphylococcus caprae
-
-
-
3.4.21.19 Staphylococcus caprae GTC 378
-
-
-
3.4.21.19 Staphylococcus cohnii subsp. cohnii
-
-
-
3.4.21.19 Staphylococcus cohnii subsp. cohnii GTC 248
-
-
-
3.4.21.19 Staphylococcus epidermidis
-
-
-
3.4.21.19 Staphylococcus epidermidis ATCC 14990
-
-
-
3.4.21.19 Staphylococcus saprophyticus
-
-
-
3.4.21.19 Staphylococcus warneri
-
-
-
3.4.21.19 Staphylococcus warneri JCM 2415
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.19 Talon affinity column chromatography Staphylococcus cohnii subsp. cohnii
3.4.21.19 Talon affinity column chromatography Staphylococcus caprae

Synonyms

EC Number Synonyms Comment Organism
3.4.21.19 GluScoh
-
Staphylococcus cohnii subsp. cohnii
3.4.21.19 GluScpr
-
Staphylococcus caprae
3.4.21.19 GluSE
-
Staphylococcus epidermidis
3.4.21.19 GluSsap
-
Staphylococcus saprophyticus
3.4.21.19 GluV8
-
Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.19 7.5
-
-
Staphylococcus aureus
3.4.21.19 7.5
-
-
Staphylococcus epidermidis
3.4.21.19 7.5
-
-
Staphylococcus saprophyticus
3.4.21.19 7.5
-
-
Staphylococcus warneri
3.4.21.19 7.5
-
-
Staphylococcus cohnii subsp. cohnii
3.4.21.19 7.5
-
-
Staphylococcus caprae