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Literature summary extracted from

  • Kalinska, M.; Kantyka, T.; Greenbaum, D.C.; Larsen, K.S.; Wladyka, B.; Jabaiah, A.; Bogyo, M.; Daugherty, P.S.; Wysocka, M.; Jaros, M.; Lesner, A.; Rolka, K.; Schaschke, N.; Stennicke, H.; Dubin, A.; Potempa, J.; Dubin, G.
    Substrate specificity of Staphylococcus aureus cysteine proteases - Staphopains A, B and C (2012), Biochimie, 94, 318-327.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.48 0.0056
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.0076
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.014
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
3.4.22.48 0.154
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.1639
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.271
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
3.4.22.48 0.385
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
3.4.22.48 0.5871
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.612
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.48 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.48 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide + H2O
-
 Staphylococcus aureus ?
-
 ?
3.4.22.48 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide + H2O
-
 Staphylococcus aureus ?
-
 ?
3.4.22.48 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide + H2O
-
 Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.48 staphopain A
-
 Staphylococcus aureus
3.4.22.48 staphopain B
-
 Staphylococcus aureus
3.4.22.48 staphopain C
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.48 37
-
 assay at Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.48 0.002
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
3.4.22.48 0.0112
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.012
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.023
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
3.4.22.48 0.032
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.056
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.089
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
3.4.22.48 0.716
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 0.886
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.48 7.6
-
 assay at Staphylococcus aureus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.48 0.1
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, value below 0.1, staphopain A Staphylococcus aureus
3.4.22.48 0.1
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, value below 0.1, staphopain C Staphylococcus aureus
3.4.22.48 0.1
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, value below 0.1, staphopain C Staphylococcus aureus
3.4.22.48 0.7
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 3.4
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 62.4
-
 2-aminobenzoyl-Ile-Ala-Lys-Asp-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain C Staphylococcus aureus
3.4.22.48 118
-
 2-aminobenzoyl-Ile-Ala-Ala-Gly-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus
3.4.22.48 127.8
-
 2-aminobenzoyl-Phe-Gly-Ala-Lys-5-amino-2-nitrobenzoylamide pH 7.6, 37°C, staphopain A Staphylococcus aureus