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Literature summary extracted from

  • Karp, F.; Harris, J.; Croteau, R.
    Metabolism of monoterpenes: Demonstration of the hydroxylation of (+)-sabinene to (+)-cis-sabinol by an enzyme preparation from sage (Salvia officinalis) leaves (1987), Arch. Biochem. Biophys., 256, 179-193.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.153 FAD exogenous, activates the enzyme slightly Salvia officinalis
1.14.13.153 FMN exogenous, activates the enzyme Salvia officinalis
1.14.13.153 octyl beta-D-glucoside 35% activation at 0.12% detergent, complete inhibition at 0.48% detergent Salvia officinalis
1.14.13.153 sodium cholate 40% activation at 0.30% detergent, 17% inhibition at 0.48% detergent Salvia officinalis
1.14.13.153 Zwittergent 3-08 12% activation at 0.24% detergent Salvia officinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.153 CO photoreversible inhibition Salvia officinalis
1.14.13.153 NADP+ NADP+ competitively inhibits P-450-dependent reactions, 40-45% inhibition of hydroxylation at 2 mM Salvia officinalis
1.14.13.153 octyl beta-D-glucoside 35% activation at 0.12% detergent, complete inhibition at 0.48% detergent Salvia officinalis
1.14.13.153 sodium cholate 40% activation at 0.30% detergent, 17% inhibition at 0.48% detergent Salvia officinalis
1.14.13.153 sodium deoxycholate 80% inhibition at 0.06% detergent Salvia officinalis
1.14.13.153 sodium taurocholate 13% inhibition at 0.30% detergent Salvia officinalis
1.14.13.153 sodium taurodeoxycholate 91% inhibition at 0.06% detergent Salvia officinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.153 microsome localization of sabinene hydroxylase in sage leaf homogenates by differential centrifugation indicated that activity is restricted to light membranes Salvia officinalis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.153 additional information no effect on enzyme activity by 1-10 mM of Mg2+, Mn2+, Ca2+, Cu2+, and Fe2+ Salvia officinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.153 (+)-sabinene + NADPH + H+ + O2 Salvia officinalis
-
 (+)-cis-sabinol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.153 Salvia officinalis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.153 epidermis
-
 Salvia officinalis
-
1.14.13.153 leaf
-
 Salvia officinalis
-
1.14.13.153 seedling etiolated Salvia officinalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.153 (+)-sabinene + NADPH + H+ + O2
-
 Salvia officinalis (+)-cis-sabinol + NADP+ + H2O
-
 ?
1.14.13.153 (+)-sabinene + NADPH + H+ + O2 the hydroxylase is highly specific for (+)-sabinene as substrate. Addition of sabinene to solubilized microsomes provides a typical type I binding spectrum Salvia officinalis (+)-cis-sabinol + NADP+ + H2O GC product analysis ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.153 20
-
 assay at Salvia officinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.153 7.4
-
-
 Salvia officinalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.153 6.8 8.6 50% of maximal activity at pH 6.8 and pH 8.6 Salvia officinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.153 NADPH
-
 Salvia officinalis

General Information

EC Number General Information Comment Organism
1.14.13.153 evolution the enzyme is a cytochrome P-450-dependent mixed function oxygenase and belongs to the cytochrome P450 oxygenase family Salvia officinalis
1.14.13.153 metabolism the enzyme catalyzes the synthesis of (+)-cis-sabinol, which is a key step in the biosynthesis of C3-oxygenated thujane monoterpenes, overview Salvia officinalis
1.14.13.153 additional information (+)-sabinene is a major olefinic constituent of the volatile oil of immature Salvia oficinalis Salvia officinalis