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Literature summary extracted from

  • Harada, H.; Shindo, K.; Iki, K.; Teraoka, A.; Okamoto, S.; Yu, F.; Hattan, J.; Utsumi, R.; Misawa, N.
    Efficient functional analysis system for cyanobacterial or plant cytochromes P450 involved in sesquiterpene biosynthesis (2011), Appl. Microbiol. Biotechnol., 90, 467-476.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.113 CYP71BA1 expression in Escherichia coli. Co-expression with germacrene A hydroxylase P450NSor CYP110C1 from Nostoc sp. PCC 7120 and the Arabidopsis thaliana ATR2 as well as two corresponding enzymes derived from Zingiber officinale, named ZoRED1 and ZoRED2 from one singel expression plasmid, construction, overview. The co-expression enhances CYP71BA1 expression levels by 1.5-2fold comapred to single heterologous expression Zingiber zerumbet

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.113 additional information reconstruction of the isoprenoid biosynthetic pathway in Escherichia coli, overview Zingiber zerumbet

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.113 Fe2+ cytochrome P450 enzyme Zingiber zerumbet

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.113 alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase] Zingiber zerumbet
-
10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.113 Zingiber zerumbet
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.113 alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
-
Zingiber zerumbet 10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.113 alpha-humulene-8-hydroxylase
-
Zingiber zerumbet
1.14.14.113 CYP71BA1
-
Zingiber zerumbet

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.113 cytochrome P450
-
Zingiber zerumbet

General Information

EC Number General Information Comment Organism
1.14.14.113 evolution the enzyme belongs to the cytochrome P450 monooxygenases Zingiber zerumbet