Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lee, J.; Paetzel, M.
    Structure of the catalytic domain of glucoamylase from Aspergillus niger (2011), Acta Crystallogr. Sect. F, 67, 188-192.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.3 industry glucoamylase from Aspergillus niger is an industrially important biocatalyst that is utilized in the mass production of glucose from raw starch or soluble oligosaccharides Aspergillus niger

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.3 purified isolated N-terminal catalytic domain of the G1 isoform, generated by subtilisin cleavage, sitting-drop vapour-diffusion method, 20 mg/ml protein at pH 8.5, 22°C room temperatur, the reservoir solution contains 50 mM Tris acetate, pH 8.5, 22.5% PEG 6000, 0.4 M sodium acetate and 10% glycerol, mixing of 0.001 ml protein and reservoir solution and equilibration against 1 ml reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.9 A resolution, the active site of the enzyme is in complex with both Tris and glycerol molecules, structure modelling Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 60000
-
 x * 60000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus niger P69328 G1 isoform
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein the linker region between starch-binding and catalytic domains is heavily glycosylated. The O-glycosylated residues are Ser467(443), Ser468(444), Thr476(452), Ser477(453), Ser483(459), Ser484(460) and Thr486(462) Aspergillus niger

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 60000, SDS-PAGE Aspergillus niger
3.2.1.3 More the G1 isoform consists of a catalytic domain and a starch-binding domain connected by a heavily glycosylated linker region Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Aspergillus niger

General Information

EC Number General Information Comment Organism
3.2.1.3 physiological function glucoamylase is an exoglucohydrolase that primarily catalyzes the hydrolysis of alpha-1,4 glycosidic linkages in raw starch and soluble oligosaccharides to generate beta-D-glucose Aspergillus niger